计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 76 篇同行评审文献引用。
概述
Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
规格
产品名称
蛋白酶K 来源于林伯氏白色念球菌
英文别名
Nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyclo[3.2.0]hept-2-ene-2-carboxylate | (4-nitrophenyl)methyl (4R,5S,6S)-6-[(1R)-1-hydroxyethyl]-4-methyl-7-oxo-3,4-diphenoxy-5-phosphonooxy-1-azabicyc
规格或纯度
EnzymoPure™, ≥20 units/mg dry weight
生化机理
蛋白酶 K 是一种稳定的高活性丝氨酸蛋白酶。晶体和分子结构研究的证据表明，该酶属于枯草蛋白酶家族，具有活性位催化三元组（Asp39-His69-Ser224）。它在 pH 值、缓冲盐、洗涤剂（SDS）和温度等多种环境中都很稳定。在 0.1-0.5% SDS 存在的情况下，蛋白酶 K 仍能保持活性，并能消化 DNA 制剂中的各种蛋白质和核酸酶，而不会影响分离 DNA 的完整性。
CAS编号和信息
39450-01-6
酶学委员会编号
3.4.21.64
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥20 units/mg dry weight
储存条件
2-8°C储存
运输条件
低温运输
单位定义
One Unit releases one micromole of Folin positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using urea denatured hemoglobin as the substrate.
技术文档
高级数据
三维结构
交互式化学结构模型
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 F2624088 |
分析证书 | P128666 |
| L2406542 |
分析证书 | P128666 |
| E2330792 |
分析证书 | P128666 |
| E2330796 |
分析证书 | P128666 |
| E2330830 |
分析证书 | P128666 |
| E2330831 |
分析证书 | P128666 |
| E2330916 |
分析证书 | P128666 |
| C2610688 |
分析证书 | P128666 |
| F1511080 |
分析证书 | P128666 |
| H2130345 |
分析证书 | P128666 |
| H2130347 |
分析证书 | P128666 |
| H2130346 |
分析证书 | P128666 |
技术文档和文章
蛋白酶K与核糖核酸酶A
目录： 技术文章
Deoxyribonuclease (DNase): DNA Hydrolytic Cleavage Properties, Methodological Value, and Multi-Context Applications
目录： 技术文章
Micrococcal Nuclease (MNase): A Technical Review of Enzymatic Properties, Reaction Control, and Research and Biomanufacturing Applications
目录： 技术文章
dsDNase-Mediated Hydrolysis of Double-Stranded DNA: Enzymatic Characteristics, Methodological Strategies, and Bioanalytical Applications
目录： 技术文章
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
目录： 技术文章
Selection Strategies for Release, Precipitation, Co-Precipitation, and Contaminant Removal Systems in Nucleic Acid Sample Pretreatment
目录： 技术文章
What Is Synthetic Biology? The Industrialization Pathway from Upstream Reagent Toolchains to Biomanufacturing Applications
目录： 技术文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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