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DNA酶I 来源于牛胰腺(过滤)

规格或纯度: ≥2,000 Kunitz units/mg dry weight
有货

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货号 (SKU) 包装规格 是否现货 价格 数量
D128598-5mg
 5mg 现货 Stock Image
D128598-25mg
 25mg 现货 Stock Image
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基本描述

别名 脱氧核糖核酸酶 I
规格或纯度 ≥2,000 Kunitz units/mg dry weight
英文名称 Deoxyribonuclease I from Bovine Pancreas(Filtered)
单位定义 1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthingto
生化机理 DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
储存温度 2-8°C储存
运输条件 冰袋运输
产品介绍

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

名称和标识符

EC号 232-667-0
酶学委员会编号 3.1.21.1
Isomeric SMILES CN(C)C1=NC(=NC(=N1)N)C(CC2=CNC3=CC=CC=C32)NC(=O)C4=CC=CC=C4
WGK Germany 3
RTECS RF0750000
PubChem CID 597667

化学和物理性质

敏感性 易潮湿

安全和危险性（GHS）

WGK Germany 3
RTECS RF0750000
个人防护装备 Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

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