计算溶液所需的质量、体积或浓度。
Mallory磷钨酸苏木素染色液 (PTAH自然氧化法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
肌纤维(Musclefiber)属于肌组织成分，由肌细胞组成，根据形态和功能特点，肌纤维可以分为平滑肌(又称横纹肌)、骨骼肌、心肌，肌纤维染色的方法有很多种，如丽春红法、苯胺蓝法、钨磷钨酸苏木素法等。最初发明磷钨酸苏木素染色液时，Mallory的PTAH方法中有多种钨磷酸苏木素方法，1900年左右Mallory将磷钨酸水溶液和苏木素染液联用，发现该法对于肌纤维染色较好，现在广泛使用的即为Mallory磷钨酸苏木素染色液(PTAH自然氧化法)，苏木素可用PTAH化学氧化法，但有效期较短且染色力易下降。尽管自然氧化耗费时间，但制得的苏木素可用2年以上，染色力也不易丢失，是较为理想的染色液，适用于CNS、一般组织结构以及所有标准固定液固定的组织，染色时间依配制方法、所用固定液和所显示的组织结构而异。
Mallory磷钨酸苏木素染色液(PTAH自然氧化法)主要由PTAH氧化剂、草酸溶液、MalloryPTAH染色液组成，MalloryPTAH染色液为自然成熟的染液，染色力较好，保存时间长，多用于显示横纹肌的横纹，临床上应用该法对横纹肌肉瘤进行诊断。横纹肌肉瘤的组织学形态变化多样，与未分化的间胚叶肿瘤很难鉴别，采用磷钨酸苏木素染色后，如果在瘤细胞胞质内发现蓝色横纹，则可以证明该肿瘤是呈横纹肌分化，该染色液也可以对炎症渗出的纤维素、DIC的毛细血管中纤维素以及神经病理等方面进行染色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| M1518515 | Component | 3×100mL | Storage |
| M1518515A | PTAH 氧化剂 A | 50mL | RT. Store in the dark. |
| M1518515B | PTAH 氧化剂 B | 50mL | RT |
| M1518515C | 草酸溶液 | 100mL | RT |
| M1518515D | Mallory PTAH 染色液 (自然氧化法) | 100mL | RT. Store in the dark. |
临用前，取A与B等量混合，即为PTAH氧化剂，即配即用。
自备材料：
1、10%福尔马林固定液、蒸馏水、系列乙醇、二甲苯或环保脱蜡透明液、中性树胶
2、光学显微镜
操作步骤(仅供参考)：
1、组织固定于10%福尔马林溶液中，常规脱水包埋。
2、石蜡切片厚4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、入新配制好的PTAH氧化剂，氧化5min，稍水洗。
4、入草酸溶液漂白1~2min，自来水冲洗2min，用蒸馏水洗1次。
5、入Mallory PTAH染色液浸染(加盖)24~48h。
6、取出切片，直接用95%乙醇迅速洗去多余染液。
7、常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
横纹肌的横纹、纤维素、胞核、红细胞和神经胶质纤维：深蓝色
胶原纤维、软骨基质：棕红色
粗的弹性纤维：紫色
注意事项：
1、在染色时若显示横纹的蓝色不够或横纹呈鲜红色，说明氧化时间不足或者可能是已过度氧化，需要重新换染液或配制新液。
2、Mallory PTAH染色后不要水洗，在95%乙醇洗时也要迅速，因为水洗或乙醇洗的时间稍长，都可以洗脱磷钨酸苏木素所着染的颜色。
3、Mallory PTAH染色液为进行性染色，不要过染，在染色24小时后可取出在显微镜下观察着色程度。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Muscle fibers are components of muscle tissue and are composed of muscle cells. According to their morphological and functional characteristics, muscle fibers can be classified into smooth muscle, skeletal muscle, and cardiac muscle. There are many staining methods for muscle fibers, such as Ponceau staining, Aniline Blue staining, and Phosphotungstic Acid Hematoxylin staining. When phosphotungstic acid hematoxylin staining solution was first developed, Mallory’s PTAH method included several variants of phosphotungstic acid hematoxylin staining. Around 1900, Mallory combined aqueous phosphotungstic acid solution with hematoxylin staining solution and found this method produced excellent staining of muscle fibers. The widely used formulation today is Mallory Phosphotungstic Acid Hematoxylin Staining Solution (PTAH, natural oxidation method). Hematoxylin can also be prepared by the PTAH chemical oxidation method, but it has a shorter shelf life and tends to lose staining potency. Although natural oxidation is time‑consuming, the resulting hematoxylin solution can be stored for more than 2 years with stable staining capacity, making it an ideal staining solution. It is suitable for tissues of the central nervous system, general histological structures, and tissues fixed with all standard fixatives. Staining time varies depending on the preparation method, fixative used, and the histological structures to be demonstrated.
Mallory Phosphotungstic Acid Hematoxylin Staining Solution (PTAH Natural Oxidation Method) mainly consists of PTAH oxidant, oxalic acid solution, and Mallory PTAH staining solution. The Mallory PTAH staining solution is naturally ripened, with good staining strength and long storage life. It is mostly used to demonstrate cross‑striations in striated muscle and is clinically applied in the diagnosis of rhabdomyosarcoma. Rhabdomyosarcoma shows diverse histological morphology and is difficult to distinguish from undifferentiated mesenchymal tumors. After phosphotungstic acid hematoxylin staining, the identification of blue cross‑striations in the cytoplasm of tumor cells confirms striated muscle differentiation. This staining solution can also be used to stain fibrin in inflammatory exudates, fibrin in capillaries in DIC, and in neuropathological examinations. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| M1518515 | Component | 3×100mL | Storage |
| M1518515A | PTAH Oxidant A | 50mL | RT. Store in the dark. |
| M1518515B | PTAH Oxidant B | 50mL | RT |
| M1518515C | Oxalic Acid Solution | 100mL | RT |
| M1518515D | Mallory PTAH Staining Solution (Natural Oxidation Method) | 100mL | RT. Store in the dark. |
Immediately before use, mix equal volumes of A and B to prepare PTAH Oxidant. Prepare and use immediately.
Materials Required (User-supplied):
1. 10% Formalin fixative, distilled water, graded ethanol, xylene or eco-friendly dewaxing clearing agent, neutral balsam
2. Light microscope
Protocol (For Reference Only):
1. Fix tissues in 10% formalin, followed by routine dehydration and embedding.
2. Prepare 4 μm paraffin sections, dewax with xylene or dewaxing clearing agent, and hydrate to water.
3. Oxidize in freshly prepared PTAH Oxidant for 5 min, then rinse briefly with water.
4. Bleach in oxalic acid solution for 1–2 min, wash in running water for 2 min, then rinse once with distilled water.
5. Stain in Mallory PTAH Staining Solution (covered) for 24–48 h.
6. Take out sections and quickly wash off excess stain with 95% ethanol.
7. Perform routine dehydration, clear with xylene or dewaxing clearing agent, and mount with neutral balsam.
Staining Results:
Cross-striations of striated muscle, fibrin, nuclei, erythrocytes, glial fibers: Deep blue
Collagen fibers, cartilage matrix: Reddish-brown
Coarse elastic fibers: Purple
Precautions:
1. If the blue color of cross-striations is weak or appears bright red during staining, it indicates insufficient or excessive oxidation. Replace the staining solution or prepare a fresh one.
2. Do not wash with water after Mallory PTAH staining, and rinse quickly with 95% ethanol. Prolonged washing with water or ethanol will remove the phosphotungstic acid hematoxylin stain.
3. Mallory PTAH Staining Solution is a progressive stain; avoid overstaining. After 24 h of staining, sections can be examined under a microscope to check staining intensity.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
Mallory三色染色试剂盒
英文别名
Mallory's Phosphotungstic Acid Hematoxylin Stain Kit (PTAH Chemical Oxidation)
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (24 months). Store in the dark.
英文名称
Mallory Phosphotungstic Acid Hematoxylin Staining Solution (PTAH Natural Oxidation Method)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书(CoA,COO,BSE/TSE 和分析图谱)
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0535344 |
分析证书 | M1518515 |
技术文档和文章
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Staining-Based Differentiation of Collagen Fibers, Muscle Fibers, and Fibrin in Tissue Sections
目录： 技术文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
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需要帮助选择级别？
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查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →相关产品
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