AKT(phospho S473) Antibody

Name: AKT(phospho S473) Antibody
SKU: Ab087899
Availability: InStock

货号: Ab087899

COA

有货

级别和纯度: ExactAb™ ? 已验证 ? 见COA

应用

IF/ICC, IHC, WB

宿主种属

兔(Rabbit)

种属反应性

人(Human), 大鼠(Rat), 小鼠(Mouse), 鸡(Chicken)

亚型

Rabbit IgG

偶联

Unconjugated

规格

库存

价格

数量

10μl

Ab087899-10μl

现货

¥374.90

50μL

Ab087899-50μl

现货

¥1,399.90

100μL

Ab087899-100μl

现货

¥1,709.90

1ml

Ab087899-1ml

期货

¥16,999.90

请输入您要添加的产品数量

大包装 / 定制

♡ 收藏夹 ⇅ 比较 ⎙ 打印页面

Skip to the end of the images gallery

Skip to the beginning of the images gallery

概述规格储存与运输图片应用技术文档高级数据关联靶点（人）质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

🧪

为什么选择此级别

ExactAb™,已验证级，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

Rabbit anti Human AKT(phospho S473) Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称

AKT(phospho S473) Antibody

别名

英文别名

规格或纯度

ExactAb™, 已验证, 见COA

宿主种属

兔(Rabbit)

特异性

AKT (phospho S473)

种属反应性

人(Human),大鼠(Rat),小鼠(Mouse),鸡(Chicken)

免疫原

A synthetic peptide derived from human AKT (phospho S473) (AA 431-480).

阳性对照

WB: HeLa, 3T3 lysates. IHC: Human breast carcinoma,Human lung, Human uterus, Mouse testis, Rat brain, Rat kidney, Rat spinal cord, Rat lung, Rat testis tissues. IF/ICC: Rat liver tissue.

偶联

Unconjugated

克隆类型

多克隆抗体

Format

Whole IgG

亚型

Rabbit IgG

纯化方法

Immunogen affinity purified

浓度

见COA

分子类型

抗体

产品线

生物产品

储存与运输

物理外观

Liquid

储存缓冲液

10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH7.2

防腐剂

0.02% sodium azide

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

图片

Western Blot: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - Western Blot
All lanes: AKT(phospho S473) Antibody (Ab087899) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 16.1 s

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899) (red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunofluorescence analysis of rat-liver tissue.
1. AKT(phospho S473) Antibody (Ab087899)(red) was diluted at 1/200 (4°C, overnight).
2. Cy3 labled Secondary antibody was diluted at 1/300 (room temperature, 50 min).
3. Picture B: DAPI (blue) 10 min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

IF/ICC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IF/ICC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AKT(phospho S473) Antibody (Ab087899). The picture on the right is blocked with the phospho peptide.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue.

1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Human-lung tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).
2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

IHC: AKT(phospho S473) Antibody (Ab087899)

AKT(phospho S473) Antibody (Ab087899) - IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue.
1. AKT(phospho S473) Antibody (Ab087899) was diluted at 1/200 (4°C, overnight).

2. Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20 min).
3. Secondary antibody was diluted at 1/200 (room temperature, 30 min). Negative control was used by secondary antibody only.

应用

应用名称

稀释比例

1/500-1/2000

IF/ICC

1/50 - 1/200

IHC

1/100-1/300

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点（人）

AKT3 Tchem RAC-γ 丝氨酸/苏氨酸蛋白激酶(RAC-gamma serine/threonine-protein kinase) (0 活性数据)

点击查看靶蛋白信息： AKT3

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

AKT2 Tchem RAC-β丝氨酸/苏氨酸蛋白激酶(RAC-beta serine/threonine-protein kinase) (0 活性数据)

点击查看靶蛋白信息： AKT2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

AKT1 Tchem RAC-alpha 丝氨酸/苏氨酸蛋白激酶(RAC-alpha serine/threonine-protein kinase) (0 活性数据)

点击查看靶蛋白信息： AKT1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到8个结果

批号(Lot Number)	证书类型	货号
ZJ25F0319278	分析证书	Ab087899
ZJ25F0319279	分析证书	Ab087899
ZJ25F0319280	分析证书	Ab087899
ZJ26F0938943	分析证书	Ab087899
ZJ26F0938942	分析证书	Ab087899
ZJ26F0938941	分析证书	Ab087899
ZJ24F0707776	分析证书	Ab087899
ZJ24F0707777	分析证书	Ab087899

技术文档和文章

磷酸化蛋白的免疫印迹法

目录：实验方案（Protocols）

探索主题:

免疫印迹蛋白质印迹 ECL 磷酸化蛋白磷酸化抗体 HRP偶联抗体

Protocol of Western blot for Phosphorylated Proteins

目录：实验方案（Protocols）

探索主题:

Western blot ECL Phosphorylated proteins Phosphorylated Antibodies Immunoblot HRP-conjugated Antibodies

蛋白质去磷酸化实验方案

目录：实验方案（Protocols）

探索主题:

SDS-PAGE 转膜碱性磷酸酶磷酸化抗体蛋白质电泳去磷酸化 AP 小牛肠碱性磷酸酶 CIP

Protein Dephosphorylation Protocol

目录：实验方案（Protocols）

探索主题:

SDS-PAGE Alkaline phosphatase Phosphorylated Antibodies Protein Electrophoresis AP CIP Dephosphorylation Calf intestinal alkaline phosphatase Transfer

AKT通路的组成、激活机制与生物学效应

目录：技术文章

探索主题:

代谢调控 GSK3 AKT PKB PI3K PTEN PDK1 mTORC2 FOXO 细胞存活

Composition, Activation Mechanisms, and Biological Effects of the AKT Pathway

目录：技术文章

探索主题:

metabolic regulation GSK3 AKT PKB PI3K PTEN PDK1 mTORC2 FOXO cell survival

Angiopoietin-TIE2信号通路的血管稳态、通透性调控与病理性血管重塑机制

目录：技术文章

探索主题:

血管生成 Angiopoietin 内皮屏障 TIE2 TEK ANGPT1 ANGPT2 TIE1

Angiopoietin-TIE2 Signaling Pathway: Mechanisms of Vascular Homeostasis, Permeability Regulation, and Pathological Vascular Remodeling

目录：技术文章

探索主题:

VEGF Angiogenesis Angiopoietin endothelial barrier Vascular permeability inflammatory microenvironment TIE2 TEK ANGPT1 ANGPT2 TIE1 pericytes tumor vessels endothelial homeostasis

ActRIIA/ActRIIB在减重中的肌肉保护与脂肪调控

目录：技术文章

探索主题:

ActRIIB Activin A ActRIIA Myostatin Bimagrumab 骨骼肌体成分

ActRIIA/ActRIIB in Weight Loss: Muscle Preservation and Adipose Regulation

目录：技术文章

探索主题:

ActRIIB Activin A ActRIIA Myostatin Bimagrumab skeletal muscle body composition

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 →

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。

AKT(phospho S473) Antibody

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

需要帮助选择级别？

查看相关“主题”系列

相关产品