Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
宿主种属
兔(Rabbit)
特异性
PRKAA1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human AMPK alpha 1 (AA 500-559).
阳性对照
WB: Raw264.7, MCF7, C6, K562, Hep G2, NIH/3T3 and HeLa cell lysates. IHC: Human kidney tissue. ICC/IF: HeLa cells.
Recombinant AMPK alpha 1 Antibody (Ab088655) - Western Blot All lanes: Recombinant AMPK alpha 1 Antibody (Ab088655) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 66 kDa Exposure time: 5.0 s
Recombinant AMPK alpha 1 Antibody (Ab088655) - IF/ICC Immunocytochemistry analysis of AMPK alpha 1 (green) in HeLa cells using Recombinant AMPK alpha 1 Antibody (Ab088655), and cells were counterstained with DAPI (blue).
Recombinant AMPK alpha 1 Antibody (Ab088655) - IHC Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Recombinant AMPK alpha 1 Antibody (Ab088655). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
应用
应用名称
稀释比例
IHC
1/100 - 1/200
IF/ICC
1/50 - 1/200
WB
1/1000 - 1/2000
高级数据
关联靶点（人）
PRKAA1Tclin5'-AMP 活化蛋白激酶催化亚基 α-1(5'-AMP-activated protein kinase catalytic subunit alpha-1)
(0 活性数据)
ZJ24F0404137
危险品化学品经营许可证（带存储）