Rabbit anti Human Carbonic Anhydrase 8/CA8 Antibody, Polyclonal, could be used for WB, IHC and so on.
Carbonic Anhydrase 8/CA8 Antibody (Ab092799) - Western Blot
All lanes: Carbonic Anhydrase 8/CA8 Antibody (Ab092799) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 38 kDa
Exposure time: 19.3 s
Carbonic Anhydrase 8/CA8 Antibody (Ab092799) - IHC
Immunohistochemical analysis of Carbonic Anhydrase 8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Carbonic Anhydrase 8/CA8 Antibody (Ab092799) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Carbonic Anhydrase 8/CA8 Antibody (Ab092799) - IF/ICC
IF analysis of Carbonic Anhydrase 8/CA8 Antibody (Ab092799) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Carbonic Anhydrase 8/CA8 Antibody (Ab092799) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/100 - 1/200
1/200
1/500-1/1000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0116420 |
分析证书 | Ab092799 |
| ZJ25F0116421 |
分析证书 | Ab092799 |
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