级别和纯度: 重组?重组（Recombinant）—— 通过重组表达生产，序列确定、批次稳定。适用于来源明确、无动物源的可重现蛋白。ExactAb™?ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。已验证?已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。Lot by Lot
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp- -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex
宿主种属
兔(Rabbit)
特异性
CASP8
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Caspase-8 (AA 385-435)
阳性对照
WB: A431, K562, A549 and THP-1 cell lysates. IHC: Human uterus tissue. ICC/IF: HeLa cells.
Recombinant Caspase-8 Antibody (Ab093061) - Western Blot All lanes: Recombinant Caspase-8 Antibody (Ab093061) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 55 kDa Observed band size: 54, 57 kDa Exposure time: 47.3 s
Recombinant Caspase-8 Antibody (Ab093061) - Western Blot All lanes: Recombinant Caspase-8 Antibody (Ab093061) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 55 kDa Observed band size: 54, 57 kDa Exposure time: 20.0 s
Recombinant Caspase-8 Antibody (Ab093061) - IF/ICC Immunofluorescence analysis of Caspase 8 in HeLa using Recombinant Caspase-8 Antibody (Ab093061).
Recombinant Caspase-8 Antibody (Ab093061) - IHC Immunohistochemistry analysis of paraffin-embedded Human uterus using Recombinant Caspase-8 Antibody (Ab093061). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
ZJ24F1215529
危险品化学品经营许可证（带存储）