Rabbit anti Human MSH6 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.
规格
产品名称
Recombinant MSH6 Antibody
别名
DNA mismatch repair protein Msh6 antibody; G/T mismatch binding protein antibody; G/T mismatch-binding protein antibody; GTBP antibody; GTMBP antibody; hMSH6 antibody; HNPCC 5 antibody; HNPCC5 antibody; HSAP antibody; MSH 6 antibody; MSH6 antibody; MSH6_H
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
宿主种属
兔(Rabbit)
特异性
MSH6
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human MSH6 (AA 1-57)
阳性对照
WB: HeLa and C6 cell lysates. IHC: Human colon carcinoma tissue. IF/ICC: HeLa cells.
Recombinant MSH6 Antibody (Ab116112) - Western Blot All lanes: Recombinant MSH6 Antibody (Ab116112) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 153 kDa Observed band size: 178 kDa Exposure time: 3.2 s
Recombinant MSH6 Antibody (Ab116112) - Western Blot All lanes: Recombinant MSH6 Antibody (Ab116112) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 153 kDa Observed band size: 178 kDa Exposure time: 2.3 s
Recombinant MSH6 Antibody (Ab116112) - IHC Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Recombinant MSH6 Antibody (Ab116112).
Recombinant MSH6 Antibody (Ab116112) - IF/ICC Immunofluorescent analysis of MSH6 in HeLa cells, using the Recombinant MSH6 Antibody (Ab116112) at 1/50 dilution.
ZJ24F1215833
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