Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
宿主种属
兔(Rabbit)
特异性
GSK3 beta
种属反应性
人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human GSK3 beta (AA 1-13)
阳性对照
WB: Jurkat, C6, CHO-K1, HeLa, MCF-7 cell lysates. IHC: Human cholangiocarcinoma tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.4
防腐剂
No
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
图片
Recombinant GSK3 beta Antibody (Ab335948) - Western Blot
All lanes: Recombinant GSK3 beta Antibody (Ab335948) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 42 kDa
Exposure time: 37.3 s
Recombinant GSK3 beta Antibody (Ab335948) - IF/ICC
Immunofluorescent analysis of HeLa cells labelling Recombinant GSK3 beta Antibody (Ab335948).
The cells were fixed with cold 100% methanol (10min, 4°C) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with Recombinant GSK3 beta Antibody (Ab335948) (1/100) at room temperature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205), (shown in green). Nuclear DNA was labeled in blue with DAPI (D609734).
Control: Secondary antibody only.
Recombinant GSK3 beta Antibody (Ab335948) - IHC
Immunohistochemical analysis of paraffin-embedded human Cholangiocarcinoma using Recombinant GSK3 beta Antibody (Ab335948).
ZJ26F0535889
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