计算溶液所需的质量、体积或浓度。
有货
Accession #
P00766
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 39 篇同行评审文献引用。
概述
Product Describtion:
Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.Three times crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized.
规格
产品名称
α-糜蛋白酶 来源于牛胰腺
别名
α-胰凝乳蛋白酶
英文别名
N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate | Chymotrypsin Substrate I, Colorimetric | N-(3-Carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide | AKOS024350585 | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]ami
规格或纯度
EnzymoPure™, ≥45 units/mg protein
生化机理
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。 α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu2+ and Hg2+.
CAS编号和信息
9004-07-3
酶学委员会编号
3.4.21.1
分子类型
酶
储存与运输
浓度
≥45 units/mg protein
储存条件
2-8°C储存
运输条件
低温运输
单位定义
One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg usi
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 K2225101 |
分析证书 | C128653 |
| E2429026 |
分析证书 | C128653 |
| K2224229 |
分析证书 | C128653 |
| K2511076 |
分析证书 | C128653 |
| B2208122 |
分析证书 | C128653 |
| I2517037 |
分析证书 | C128653 |
| H2217137 |
分析证书 | C128653 |
| H2217195 |
分析证书 | C128653 |
| H2217198 |
分析证书 | C128653 |
| L2410148 |
分析证书 | C128653 |
| E1528104 |
分析证书 | C128653 |
技术文档和文章
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
目录： 技术文章
Review of Chymotrypsin: Enzymological Properties, Catalytic Mechanism, and Research Applications
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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