Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.

规格

产品名称

α-糜蛋白酶来源于牛胰腺(纯化)

别名

α-胰凝乳蛋白酶

英文别名

N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate | Chymotrypsin Substrate I, Colorimetric | N-(3-Carboxy-1-oxopropyl)glycylglycyl-N-(4-nitrophenyl)-L-phenylalaninamide | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acid | CHYMOTRYPSIN | 4-Hydroxy-4-({2-hydroxy-2 展开

规格或纯度

EnzymoPure™, ≥45 units/mg protein

生化机理

一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。 α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

CAS编号和信息

9004-07-3

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥45 units/mg protein

储存条件

2-8°C储存

运输条件

低温运输

高级数据

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批号(Lot Number)	证书类型	货号
L2220159	分析证书	C129080
F1501086	分析证书	C129080

技术文档和文章

蛋白样品制备流程

目录：技术文章

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蛋白样品制备

Protein sample preparation process

目录：技术文章

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Protein sample preparation

先进的酶分析技术

目录：技术文章

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生物制药酶催化酶活性酶分析技术

蛋白质分子量标准

目录：技术文章

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蛋白质蛋白质分子量标准

Protein Molecular Weight Standards

目录：技术文章

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protein Protein Molecular Weight Standards

抑肽酶技术综述：结构特征、作用机制与应用

目录：技术文章

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抑肽酶

A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications

目录：技术文章

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Aprotinin

科研常用蛋白酶的基础介绍与典型应用

目录：技术文章

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蛋白酶蛋白质组学肽键水解底物特异性受限水解抗体片段化去蛋白处理工业酶制剂

Commonly Used Proteases in Research: Fundamentals and Representative Applications

目录：技术文章

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Proteomics proteases peptide-bond hydrolysis substrate specificity limited proteolysis antibody fragmentation deproteinization industrial enzyme preparations

糜蛋白酶（Chymotrypsin）的酶学特性、催化机制与科研应用综述

目录：技术文章

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肽图丝氨酸蛋白酶底物特异性催化三联体糜蛋白酶 α-糜蛋白酶氧阴离子孔酰酶中间体限制性蛋白水解方法学质控

Review of Chymotrypsin: Enzymological Properties, Catalytic Mechanism, and Research Applications

目录：技术文章

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Peptide Mapping Serine protease substrate specificity limited proteolysis catalytic triad Chymotrypsin Alpha-Chymotrypsin Oxyanion Hole Acyl-Enzyme Intermediate Methodological Quality Control

IHC实验中低pH、高pH及酶法抗原修复体系的应用比较

目录：技术文章

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IHC 抗原修复 FFPE 低 pH 热修复高 pH 热修复酶法修复

Comparison of Low-pH, High-pH, and Enzymatic Antigen Retrieval Systems in IHC

目录：技术文章

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IHC Antigen Retrieval FFPE low-pH heat retrieval high-pH heat retrieval enzymatic retrieval

Advanced enzyme analysis technology

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Pharmaceuticals Enzyme catalysis enzymatic activity Enzyme analysis technology

内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用

目录：技术文章

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蛋白质降解 Endopeptidase 氨基肽酶外肽酶 exopeptidase 内肽酶肽段加工羧肽酶二肽基肽酶

Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research

目录：技术文章

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Protein degradation Endopeptidase aminopeptidase exopeptidase Peptide Processing carboxypeptidase dipeptidyl peptidase

生物模拟肽的皮肤作用逻辑：序列与构象、皮肤递送、稳定性及靶点匹配

目录：技术文章

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脂质体生物模拟肽序列仿生信号肽角质层递送脂质化修饰蛋白酶稳定性穿胞肽细胞靶点匹配细胞外基质调节

The Logic of Biomimetic Peptide Action in Skin: Sequence and Conformation, Skin Delivery, Stability, and Target Matching

目录：技术文章

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liposomes Cell-Penetrating Peptides biomimetic peptides sequence mimicry signaling peptides stratum corneum delivery lipidation proteolytic stability cellular target matching extracellular matrix regulation

丝氨酸内切蛋白酶的催化机制、分类特征与实验应用

目录：技术文章

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胰蛋白酶丝氨酸蛋白酶凝血酶催化三联体蛋白水解丝氨酸内切蛋白酶 EC 3.4.21 枯草杆菌蛋白酶

Catalytic Mechanisms, Classification Characteristics, and Experimental Applications of Serine Endopeptidases

目录：技术文章

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Trypsin Thrombin Subtilisin catalytic triad proteolysis EC 3.4.21 serine endopeptidases serine proteases

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