DNA酶I

CAS: 9003-98-9 货号: D128591 EC号: 232-667-0
有货
级别和纯度: EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 来源于牛胰腺;≥2,000 units/mg dry weight;无Ribonuclease & Protease
Accession #
P00639
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规格
库存
价格
数量
2.5KU
D128591-2.5KU
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¥694.90
10KU
D128591-10KU
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¥2,522.90
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为什么选择此级别

EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 45 篇同行评审文献引用。

概述

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.

规格

产品名称
DNA酶I
别名
脱氧核糖核酸酶 I
规格或纯度
EnzymoPure™, 来源于牛胰腺;≥2,000 units/mg dry weight;无Ribonuclease & Protease
生化机理
DNase I 是一种内切酶,可作用于嘧啶附近的磷酸二酯键,生成末端带有 5′-磷酸的多核苷酸。在 Mg2+ 存在的情况下,DNAse I 可独立裂解 DNA 的每一条链,裂解位点是随机的。在 Mn2+ 存在的情况下,两条 DNA 链会在大致相同的部位被裂解。Mn2+、Ca2+、Co2+ 和 Zn2+ 等二价阳离子是该酶的活化剂。浓度为 5 mM 的 Ca2+ 能使酶稳定,防止蛋白水解。最佳 pH 值为 7 至 8。牛胰腺中的 DNAse I 由 A、B、C 和 D 四种色谱法可区分的成分组成,它们的摩尔比分别为 4:1:1。其中只有少量的 D。已知 2-巯基乙醇、螯合剂、十二烷基硫酸钠(SDS)6 和肌动蛋白7 可抑制酶的活性。
CAS编号和信息
9003-98-9
分子类型
酶
储存与运输
浓度
来源于牛胰腺;≥2,000 units/mg dry weight;无Ribonuclease & Protease
储存条件
2-8°C储存
运输条件
低温运输

技术文档

📋 安全数据表 (SDS)

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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找到5个结果

批号(Lot Number) 证书类型 货号
H2614076 分析证书 D128591
F1723048 分析证书 D128591
L2413187 分析证书 D128591
L2413215 分析证书 D128591
H1517086 分析证书 D128591
技术文档和文章
CRISPR-Cas9和靶向基因组编辑
CRISPR/Cas9 and targeted genome editing
流式实验样品制备实验指南
Experimental Guide of Sample Preparation for Flow Cytometry
CUT&RUN-seq实验方案
RNA提取和反转录实验方案
RNA Extraction and Reverse Transcription Protocol
如何选择血浆游离RNA提取试剂
How to Choose Plasma Cell-Free RNA (cfRNA) Extraction Reagents
多种组织单细胞解离酶的应用与优化
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
常见组织解离酶的原理与选择策略
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
His-Tag蛋白纯化时常见问题及解决方法
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
Proteinase K常见问题
Proteinase K: Frequently Asked Questions
植物组学技术全景:基因组—转录组—蛋白质组—代谢组的流程框架与多组学整合应用
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
RNA提取技术:方法体系、操作流程与质量控制要点
RNA Extraction Technologies: Method Systems, Operational Workflows, and Key Quality-Control Considerations
脱氧核糖核酸酶(DNase):DNA水解切割特性、方法学价值与多场景应用
Deoxyribonuclease (DNase): DNA Hydrolytic Cleavage Properties, Methodological Value, and Multi-Context Applications
分散酶(Dispase):基底膜松解型组织解离蛋白酶的性质、制备与应用体系
白介素29(IL-29/IFN-λ1):分子特征、信号网络与科研应用体系
Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications
二苯胺的理化特性、分析应用及其与DNA显色反应综述
Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction
胶原酶(Collagenase)的酶学特征、类型体系与科研应用综述
Review of Collagenase: Enzymological Features, Type Systems, and Research Applications
梭菌蛋白酶的类型谱系、分子机制与科研应用综述
Clostridial Proteases: Classification, Molecular Mechanisms, and Research Applications
组织单细胞制备的样本处理与质控策略
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
细胞样本保存体系对形态保持、抗凝稳定与检测结果的影响
Effects of Cell Sample Preservation Systems on Morphological Integrity, Anticoagulant Stability, and Analytical Results
细胞凋亡检测中的TUNEL标记与结果判读
Cell Apoptosis Detection by TUNEL Labeling: Signal Interpretation and Result Evaluation
CUT&RUN-seq protocol
PDRN参与皮肤修复的机制:A2A受体相关信号、核苷酸补救与细胞外基质重塑
Mechanisms by Which PDRN Participates in Skin Repair: A2A Receptor-Related Signaling, Nucleotide Salvage, and Extracellular Matrix Remodeling
此产品的引用文献
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