序列相似性：Belongs to the peptidase C1 family.
木瓜蛋白酶是采用生物工程技术从番木瓜植物未成熟的果实中提炼而得的纯天然生物酶制品，它是由212个氨基酸组成，分子量为21000，属于含巯基（—SH）肽链内切酶，具有蛋白酶和酯酶的活性，有较广泛的特异性，对动植物蛋白、多肽、酯、酰胺等有较强的酶解能力，同时还具有合成的能力，可把蛋白质水解物再合成蛋白质类物质，这种能力可用来改善动植物蛋白的营养价值或功能性质。
产品特点：
1、采用先进的生物工程技术、膜分离技术及真空冷冻干燥技术，并成功开发专有酶保护技术，减少了加工过程中酶活力的损失。
2、以美国药典标准及中国现行标准组织生产，依照国际质量认证体系要求，建立严格的质量控制体系，严格控制产品中微生物，达到国家出口食品卫生标准。
3、解决超滤技术在木瓜蛋白酶上的高效分离，在常温下提取木瓜蛋白精酶，木瓜浆蛋白酶回收率达90%以上，提高了酶的回收率，降低成本。
产品性状：淡黄色或白色固体粉末
应用领域：
1、食品工业：
利用木瓜蛋白酶的酶促反应，可把食品中大分子的蛋白质水解成易于吸收的小分子肽或氨基酸，广泛适用于如：鸡、猪、牛、海产品、血制品、大豆、花生等动植物蛋白酶解、肉类嫩化剂、酒类澄清剂、饼干松化剂、面条稳定剂、保健食品、酱油酿造及酒类发酵剂等，即可提高食品的营养价值，又可降低成本。
2、饼干行业：
降低面团湿筋度、改良面团可塑性及理化性质，同时使蛋白质大分子水解成短肽和氨基酸，从而有利于糖类和氨基类物质进行复杂的美拉德反应，使产品上色快，色泽悦目有油润光鲜感，疏松酥脆容比力大，断面网状结构明显，层次好；裂饼、碎饼率降低，饼形端正饱满不缩身，花纹清晰，饼面光滑；并且可以相应减少10％-25％的焦亚硫酸钠，从而减少SO2等有害物质的残留量，还可以矫正化学添加剂对饼干风味的影响，有效提高饼干产品的质量。
3、医药工业：
含木瓜蛋白酶的药物，如木瓜润喉片、木瓜肠溶片（胶囊）、木瓜含片，具有消炎、利胆、止痛、助消化、提高免疫力等功效，进一步研究表明亦可用于检查红细胞表面及血型鉴别剂、治疗妇科病、青光眼、昆虫的叮咬等。
4、纺织工业：
羊毛的防缩：用木瓜蛋白酶处理过的羊毛，其抗张度比常规方法高，毛线手感柔软，舒适、抗
收缩、抗张强度等效果收缩性为0；还可用于蚕的脱胶和蚕丝的精炼。
5、皮革工业：
利用木瓜蛋白酶制成皮的脱毛剂，鞣制皮革，经本品鞣制的皮革，毛孔细致光亮。
6、饲料工业：
分解饲料中蛋白质成氨基酸，增加营养物质的渗出量，利于家禽对蛋的吸收和利用，同时补充动物内源酶不足，提高饲料利用率及降低饲料成本，增强食欲，进而促进动物生长，提高日增重及成活力，还可以作为蔬菜水果高级复合肥料的添加剂。
7、日化行业：
用于香皂、肥皂、洗涤剂、洗衣粉、洗手液等，衣物被血渍,牛奶,果汁,酱油油脂等污染后,如用普通洗涤剂,则一般很难消除这些污渍。如若在洗涤剂中加入蛋白酶可使汗渍,血渍容易除去，去污力强，除菌消毒、安全放心。
8、化妆品行业：
木瓜酶作用于人体皮肤老化角质层，促使其分解退化、去除达到嫩肤效果及促进细胞生长的效果，且木瓜酶水解物在皮肤表层形成一层氨基酸衍物薄膜，使肌肤保持润湿与光滑；木瓜酶易于与形成色斑的黑色素中的铜离子形成络合物，减少黑色素的形成和去除色斑的黑色素，且木瓜酶水解的三肽物质可直接抑制形成黑色素的酪氨酸活性、消除自由基作用，从而达到美白去斑的效果
9、 此外还可加入加牙膏、漱口水、牙粉等,可清洁口腔，除去牙垢和牙结石，与其它溶剂配合可制成隐形眼镜的清洁消毒剂以及感光胶片银的回收等。
使用条件：
最适温度：50—55℃
最适 pH值：6.5
酶的最适添加量必须按照具体生产情况而定。
储存：
建议在阴凉干燥的环境下避光保存。
贮藏过久或贮藏条件不利，会使酶活不同程度的降低；如温度湿度过高，则需要在使用时适当的增加使用量。
安全：
木瓜蛋白酶属于纯天然的酶制剂，是蛋白质，食用酶制剂的食品如同食用含有蛋白质类食物一般，对人体有益无害。
对于部分敏感人群，如直接摄入高浓缩的酶粉或雾滴，有可能引起过敏，过长时间接触有可能刺激皮肤、眼睛和粘膜组织。在操作过程中建议配戴口罩、眼罩等防护用具，剩余或洒出的酶粉需及时处理，对于大量洒出的酶粉应轻轻扫回容器，少量则用真空吸走或用水浸湿清理。
木瓜蛋白酶是一种生物活性物质，易受重金属离子（Fe³⁺, Cu²⁺, Hg⁺, Pb⁺等）和氧化剂的抑制及破坏作用，在贮存或使用过程中应避免与之接触。
Papain is a 100% natural bioenzyme product extracted from the unripe fruits of Carica papaya L. via advanced bioengineering technologies. Composed of 212 amino acid residues with a molecular weight of 21,000 Da, it is classified as a thiol (-SH)-containing endopeptidase. Endowed with both proteolytic and esterolytic activities, papain exhibits broad substrate specificity—exerting potent hydrolytic effects on animal/plant proteins, polypeptides, esters, and amides. Notably, it also possesses synthetic capability, enabling the resynthesis of proteinaceous substances from protein hydrolysates. This unique property can be harnessed to enhance the nutritional value or functional characteristics of animal and plant proteins.
Product Features
1. Advanced Processing Technologies: Integration of state-of-the-art bioengineering, membrane separation, and vacuum freeze-drying technologies, coupled with proprietary enzyme stabilization technology, minimizes enzyme activity loss during manufacturing.
2. Stringent Quality Compliance: Manufactured in accordance with United States Pharmacopeia (USP) standards and current Chinese national standards. A rigorous quality control system, aligned with international quality certification requirements, ensures strict microbial limits, meeting the national hygiene standards for export food.
3. High-Efficiency Ultrafiltration Separation: Optimized ultrafiltration technology for papain purification enables the extraction of high-purity papain under ambient temperature conditions, achieving a protease recovery rate of over 90%. This significantly improves yield and reduces production costs.
Product Appearance: Pale yellow or white solid powder
Application Fields
1. Food Industry
Leveraging its enzymatic catalysis, papain hydrolyzes macromolecular proteins in food into small-molecule peptides and amino acids for easy absorption. It is widely used in:
Hydrolysis of animal/plant proteins (chicken, pork, beef, seafood, blood products, soybean, peanut, etc.)
Meat tenderizer, wine clarifier, biscuit crisping agent, noodle stabilizer
Health food production, soy sauce brewing, and wine fermentation starter
Papain not only enhances the nutritional value of food but also reduces production costs.
2. Biscuit Industry
Reduces dough gluten viscosity, improves dough plasticity and physicochemical properties.
Hydrolyzes macromolecular proteins into short peptides and amino acids, facilitating the Maillard reaction between sugars and amino compounds—resulting in faster color development, a bright, glossy, and appetizing appearance, enhanced crispness, and a distinct reticular cross-section with clear layers.
Lowers biscuit cracking and breakage rates, yielding well-shaped, plump, non-shrinking biscuits with sharp patterns and smooth surfaces.
Reduces the usage of sodium metabisulfite by 10%–25%, thereby decreasing residual harmful substances such as SO₂. It also mitigates the negative impact of chemical additives on biscuit flavor, significantly improving product quality.
3. Pharmaceutical Industry
Drugs formulated with papain (e.g., papain throat lozenges, enteric-coated tablets/capsules, buccal tablets) exhibit anti-inflammatory, cholagogic, analgesic, digestive-aiding, and immunity-boosting effects. Further research indicates its potential applications in:
Red blood cell surface analysis and blood group identification
Treatment of gynecological disorders, glaucoma, and insect bites
4. Textile Industry
Wool Shrink-Resistant Finishing: Wool treated with papain demonstrates higher tensile strength than conventionally processed wool, with a soft, comfortable handfeel and zero shrinkage.
Silk Degumming and Refining: Effective for degumming raw silk and refining silk fibers.
5. Leather Industry
Papain-based depilatory agents are used in leather tanning processes, producing leather with fine, smooth, and glossy pores.
6. Feed Industry
Hydrolyzes feed proteins into amino acids, increasing nutrient solubility and improving absorption and utilization in poultry.
Supplements endogenous enzyme deficiencies in animals, enhancing feed conversion efficiency and reducing feed costs.
Stimulates appetite, promotes animal growth, and increases daily weight gain and survival rates.
Can be used as an additive in high-grade compound fertilizers for fruits and vegetables.
7. Daily Chemical Industry
Incorporated into soaps, detergents, laundry powders, and hand sanitizers, papain effectively removes stubborn stains such as blood, milk, fruit juice, soy sauce, and grease—stains that are typically difficult to eliminate with ordinary detergents. It enhances detergency, provides bactericidal and disinfectant effects, and ensures safe use.
8. Cosmetic Industry
Skin Rejuvenation: Acts on aged cuticle cells, promoting their decomposition and exfoliation to achieve a skin-smoothing effect and stimulate cell proliferation. Papain hydrolysates form a film of amino acid derivatives on the skin surface, maintaining skin hydration and smoothness.
Whitening and Spot Lightening: Easily chelates copper ions in melanin (the key pigment causing skin spots), reducing melanin synthesis and fading existing spots. Additionally, tripeptides derived from papain hydrolysis directly inhibit the activity of tyrosinase (the enzyme responsible for melanin production) and scavenge free radicals, thereby achieving whitening and spot-removing effects.
9. Other Applications
Added to toothpaste, mouthwash, and tooth powder for oral hygiene, effectively removing dental plaque and tartar.
Formulated with other solvents to produce cleaning and disinfecting agents for contact lenses.
Used in the recovery of silver from photographic films.
Recommended Application Conditions
Optimum Temperature: 50–55℃
Optimum pH: 6.5
Optimum Dosage: Must be adjusted according to specific production conditions.
Storage Instructions
Store in a cool, dry, and dark place. Prolonged storage or adverse storage conditions (e.g., high temperature and humidity) may cause varying degrees of enzyme activity loss. In such cases, the dosage should be appropriately increased during application.
Safety Precautions
As a natural enzyme preparation and protein, papain is safe for human consumption—equivalent to consuming protein-rich foods—and offers nutritional benefits.
High-concentration papain powder or mists may trigger allergic reactions in sensitive individuals. Prolonged contact may cause irritation to the skin, eyes, and mucous membranes. Wear protective equipment such as masks and goggles during operation. Promptly clean up any excess or spilled enzyme powder: sweep large spills gently back into containers; use a vacuum cleaner or damp cloth to clean small spills.
Papain is a biologically active substance susceptible to inhibition or inactivation by heavy metal ions (Fe³⁺, Cu²⁺, Hg⁺, Pb⁺, etc.) and oxidizing agents. Avoid contact with these substances during storage and application.
I2623007
危险品化学品经营许可证（带存储）