BDNF Antibody

货号: Ab090903
有货
级别和纯度: ExactAb™ ? ExactAb™ —— 阿拉丁强调精准和验证质量的抗体系列。适用于可靠、特异性经验证的抗体应用。 已验证 ? 已验证级 —— 发布前在特定应用中确认性能。当需要试剂按声称工作的书面保证时使用。 1.0 mg/mL
应用
IF/ICC, IHC, WB
宿主种属
兔(Rabbit)
种属反应性
人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型
Rabbit IgG
偶联
Unconjugated
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规格
库存
价格
数量
10μl
Ab090903-10μl
现货 Stock Image
¥374.90
50μL
Ab090903-50μl
现货 Stock Image
¥974.90
100μL
Ab090903-100μl
现货 Stock Image
¥1,574.90
1ml
Ab090903-1ml
期货 Stock Image
¥16,999.90
请输入您要添加的产品数量
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为什么选择此级别

ExactAb™,已验证 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Rabbit anti Human BDNF Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

规格

产品名称
BDNF Antibody
别名
脑源性神经营养因子(BDNF)多克隆抗体 | BDNF抗体 | BDNF 抗体 | 脑源性神经营养因子(BDNF)多克隆抗体 | BDNF抗体
英文别名
BDNF | ANON2 | BULN2 | Abrineurin antibody | ANON2 antibody | BDNF antibody | BDNF_HUMAN antibody | Brain Derived Neurotrophic Factor antibody | Brain-derived neurotrophic factor antibody | BULN2 antibody | MGC34632antibody | Neurotrophinantibody | BDNF R
规格或纯度
ExactAb™, Validated, 1.0 mg/mL
生化机理
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
宿主种属
兔(Rabbit)
特异性
BDNF
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human BDNF (AA 150-200).
阳性对照
WB: Mouse heart, HeLa, HEK293, THP-1, K562, Hep G2, RAW 264.7 and C6 lysates. IHC: Human brain tissue. IF/ICC: HeLa cell.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
图片

BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 17, 28, 30 kDa
BDNF is synthesized as a precursor form (pro-BDNF) and is converted into its active form through proteolytic processing. The precursor protein is first cleaved to pro-BDNF (~35 kDa) by removing a signal peptide, and then to a mature form of BDNF (~15 kDa). Additionally, pro-BDNF can form dimers that run between 50 -60 kDa, and a minor truncated form of pro-BDNF running at ~28 kDa has also been reported (PMID: 11152678, 20630543).
Exposure time: 3.2 s

BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 16, 28, 30, 57 kDa
BDNF is synthesized as a precursor form (pro-BDNF) and is converted into its active form through proteolytic processing. The precursor protein is first cleaved to pro-BDNF (~35 kDa) by removing a signal peptide, and then to a mature form of BDNF (~15 kDa). Additionally, pro-BDNF can form dimers that run between 50 -60 kDa, and a minor truncated form of pro-BDNF running at ~28 kDa has also been reported (PMID: 11152678, 20630543).
Exposure time: 44.7 s
 

BDNF Antibody (Ab090903) - IF/ICC
Immunofluorescence analysis of BDNF in HeLa using BDNF Antibody (Ab090903), and cells were counterstained with DAPI (blue).

BDNF Antibody (Ab090903) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using BDNF Antibody (Ab090903). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用
应用名称
稀释比例
IF/ICC

1/50-1/200

IHC

1/100 - 1/200

WB

1/1000 - 1/2000

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

关联靶点(人)
BDNF Tchem 脑源性神经营养因子(Brain-derived neurotrophic factor) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
作用机制
作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到6个结果

批号(Lot Number) 证书类型 货号
ZJ24F0303140 分析证书 Ab090903
ZJ24F0303141 分析证书 Ab090903
ZJ25F0116744 分析证书 Ab090903
ZJ25F0116743 分析证书 Ab090903
ZJ25F0116742 分析证书 Ab090903
ZJ24F1215875 分析证书 Ab090903
技术文档和文章
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
免疫组织化学染色的抗原修复方案
免疫肽封闭实验
Immunopeptide blocking assay
免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Immunohistochemistry (IHC) staining antigen retrieval protocol
神经可塑性研究中的分子指标:BDNF/TrkB、CREB磷酸化与突触蛋白检测
Molecular Markers in Neuroplasticity Research: BDNF/TrkB, CREB Phosphorylation, and Synaptic Protein Detection
阿尔茨海默病核心病理靶点的分子机制、生物标志物价值与靶向治疗进展
Molecular Mechanisms, Biomarker Value, and Advances in Targeted Therapy for Core Pathological Targets in Alzheimer’s Disease
细胞衰老的分子基础与抗衰老干预研究
Molecular Basis of Cellular Senescence and Anti-Aging Intervention Research
溶液计算器

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