Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
宿主种属
兔(Rabbit)
特异性
TFE3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human TFE3 (AA 150-450)
阳性对照
WB: HeLa, C2C12 and C6 cell lysates. IHC: Human bladder tissue. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant TFE3 Antibody (Ab130766) - Western Blot All lanes: Recombinant TFE3 Antibody (Ab130766) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 62 kDa Observed band size: 63 kDa Exposure time: 65.0 s
Recombinant TFE3 Antibody (Ab130766) - Western Blot All lanes: Recombinant TFE3 Antibody (Ab130766) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 62 kDa Observed band size: 63 kDa Exposure time: 65.0 s
Recombinant TFE3 Antibody (Ab130766) - Western Blot All lanes use the Recombinant TFE3 Antibody (Ab130766) at 1/1000 dilution for 1 hour at room temperature.
Recombinant TFE3 Antibody (Ab130766) - IHC Immunohistochemical analysis of paraffin-embedded human bladder, using Recombinant TFE3 Antibody (Ab130766).
Recombinant TFE3 Antibody (Ab130766) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant TFE3 Antibody (Ab130766) at 1/50 dilution.
Recombinant TFE3 Antibody (Ab130766) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant TFE3 Antibody (Ab130766) at 1/150 dilution.