α-糜蛋白酶(质谱级)

CAS: 9004-07-3 货号: C1455924 EC号: 232-671-2 PubChem CID: 4052643
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 质谱级 (MS) ? 适用于质谱(MS)—— 非挥发性和离子残留低,与质谱兼容。用于在质谱流程中尽量降低背景和离子抑制。 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 冻干粉 ? 冻干粉 —— 温和冷冻干燥以保持活性、便于储存。适用于使用前复溶的敏感生物制品。 用于蛋白测序 ? 蛋白测序级 —— 供 Edman/质谱测序的超洁净试剂。当污染物会掩盖序列测定时使用。 来源于牛胰腺; ≥30 U/mg protein (实测值见COA)
Accession #
P00766,P00767
生物活性
≥30U/mg protein
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规格
库存
价格
数量
50μg
C1455924-50μg
期货 Stock Image
¥999.90
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为什么选择此级别

EnzymoPure™,冻干粉,天然,无载体,生物活性,用于蛋白测序,质谱级 (MS) 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 38 篇同行评审文献引用。

概述

  胰凝乳蛋白酶是来源于牛胰的一种丝氨酸蛋白酶,可特异性酶切Tyr、Phe、Trp和Leu的C-末端的肽键。也有少量的Met、Ala、Asp和Glu的C-末端的肽键裂解。其中的残留胰蛋白酶用TLCK灭活后纯化处理。该蛋白酶用Ca²⁺离子激活并稳定

  pH 值范围:在PH=7.8时,α-Chymotrypsin蛋白酶具有最佳活性。

  适用范围:α-Chymotrypsin蛋白酶特异性水解变性后蛋白质Y、F、W和L位。

溶液酶解SOP:

  胰凝乳蛋白酶按照1:50蛋白酶和蛋白的量(w/w)使用,在30℃下于含10mM CaCl₂,pH7.8的100mM Tris-HCI中进行酶切消化2-12小时。

注意事项:

1.水浴温度高于37°C时蛋白酶会自我水解。

2.样品消化过程中尿素和盐酸胍的浓度最好小于1M。


规格

产品名称
α-糜蛋白酶(质谱级)
别名
胰凝乳蛋白酶 | 糜蛋白酶
英文别名
chymotrypsin | alpha-chymotrypsin | chymotrypsin A | chymotrypsin B
规格或纯度
无载体, 生物活性, 质谱级 (MS), 天然, EnzymoPure™, 冻干粉, 用于蛋白测序, 来源于牛胰腺; ≥30 U/mg protein (实测值见COA)
生物活性
≥30U/mg protein
种属
牛(Bovine)
无载体
Yes
无动物源
No
Accession #
来源
天然
预测分子量
25 kDa
CAS编号和信息
9004-07-3
酶学委员会编号
3.4.21.1
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 0.1%TFA, 5mM CaCl2, pH3.0
浓度
来源于牛胰腺; ≥30 U/mg protein (实测值见COA)
复溶
用超纯水复溶,配制成 0.5 mg/mL 的浓度。复溶后于‑20℃条件下可保存 3 个月。
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(2年); 避免反复冻融。

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到1个结果

批号(Lot Number) 证书类型 货号
ZJ25F0926445 分析证书 C1455924
技术文档和文章
简介:“适用于质谱分析” (又名:质谱级)
Introduction: “Suitable for Mass Spectrometry”
纯净提取,智慧溶剂:天然提取试剂实践手册
Clean Extracts, Smart Solvents: The Natural Extraction Reagent Playbook
用于蛋白测序
For Protein Sequencing
质谱级 (MS)
MS Grade
质谱级酶:精准蛋白质组学分析的“分子剪刀”
MS-Grade Enzymes:“Molecular Scissors” for Precision Proteomics Analysis
蛋白质分子量标准
Protein Molecular Weight Standards
抑肽酶技术综述:结构特征、作用机制与应用
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
科研常用蛋白酶的基础介绍与典型应用
Commonly Used Proteases in Research: Fundamentals and Representative Applications
糜蛋白酶(Chymotrypsin)的酶学特性、催化机制与科研应用综述
Review of Chymotrypsin: Enzymological Properties, Catalytic Mechanism, and Research Applications
IHC实验中低pH、高pH及酶法抗原修复体系的应用比较
Comparison of Low-pH, High-pH, and Enzymatic Antigen Retrieval Systems in IHC
内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
生物模拟肽的皮肤作用逻辑:序列与构象、皮肤递送、稳定性及靶点匹配
The Logic of Biomimetic Peptide Action in Skin: Sequence and Conformation, Skin Delivery, Stability, and Target Matching
丝氨酸内切蛋白酶的催化机制、分类特征与实验应用
Catalytic Mechanisms, Classification Characteristics, and Experimental Applications of Serine Endopeptidases
此产品的引用文献
引用文献
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溶液计算器

常见问题

本产品应如何储存?
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。