脱氧核糖核酸酶 I 来源于牛胰腺

CAS: 9003-98-9 货号: D755014 EC号: 232-667-0
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级别和纯度: Type IV, lyophilized powder,≥2,000 Kunitz units/mg protein
Accession #
P00639
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规格
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数量
15KU
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375KU
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为什么选择此级别

,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 45 篇同行评审文献引用。

概述

DNAse I可用于切开DNA,作为将标记碱基插入DNA的第一步。来自sigma的该酶已用于处理大鼠的脑组织。该研究表明星形胶质细胞的轴突生长不受少突胶质细胞抑制。在另一项研究中,大肠杆菌的解冻固定样本被来自Sigma的DNAseI以及其他酶进行消化。消化是在进行通透化处理和核酸染色前完成的。来自牛胰腺的脱氧核糖核酸酶I已用于研究枯草芽孢杆菌中核酸酶的二维酶谱分析。来自牛胰腺的脱氧核糖核酸酶I还用于研究小沟和大沟结合药物和嵌入剂对小沟结合蛋白RecA和脱氧核糖核酸酶I的DNA结合的影响。用于从蛋白质样品中除去 DNA。

DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes. In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids.
Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
Used for the removal of DNA from protein samples.

规格

产品名称
脱氧核糖核酸酶 I 来源于牛胰腺
规格或纯度
Type IV, lyophilized powder,≥2,000 Kunitz units/mg protein
生化机理
DNase I是一种内切核酸酶,可作用于与嘧啶相邻的磷酸二酯键以产生具有末端5′-磷酸的聚核苷酸。当Mg2+存在时,DNAse I可独立切割DNA的每条链且切割位点是随机的。当Mn2+存在时,两条DNA链都几乎是在同样的位置被切割。最适pH在7-8之间。二价阳离子如Mn2+, Ca2+, Co2+以及Zn2+都是酶的激活剂。5 mM浓度的Ca2+可稳定酶免收蛋白水解酶的消化。来自牛胰腺的DNAse I由四种色谱可区分的组分A,B,C和D组成,它们的摩尔比为4:1:1。仅有少量的D被发现。2-巯基乙醇、螯合剂、十二烷基硫酸钠(SDS)和肌动蛋白都已知可抑制酶的活性。
CAS编号和信息
9003-98-9
酶学委员会编号
3.1.21.1(BRENDA,IUBMB)
分子类型
酶
储存与运输
物理形态
固体
浓度
Type IV, lyophilized powder,≥2,000 Kunitz units/mg protein
储存条件
-20°C储存
运输条件
超低温运输

技术文档

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
多种组织单细胞解离酶的应用与优化
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
常见组织解离酶的原理与选择策略
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
His-Tag蛋白纯化时常见问题及解决方法
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
RNA Pull-Down实验操作规程
RNA Pull-Down Standard Operating Procedure (SOP)
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植物组学技术全景:基因组—转录组—蛋白质组—代谢组的流程框架与多组学整合应用
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
RNA提取技术:方法体系、操作流程与质量控制要点
RNA Extraction Technologies: Method Systems, Operational Workflows, and Key Quality-Control Considerations
脱氧核糖核酸酶(DNase):DNA水解切割特性、方法学价值与多场景应用
Deoxyribonuclease (DNase): DNA Hydrolytic Cleavage Properties, Methodological Value, and Multi-Context Applications
分散酶(Dispase):基底膜松解型组织解离蛋白酶的性质、制备与应用体系
白介素29(IL-29/IFN-λ1):分子特征、信号网络与科研应用体系
Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications
二苯胺的理化特性、分析应用及其与DNA显色反应综述
Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction
胶原酶(Collagenase)的酶学特征、类型体系与科研应用综述
Review of Collagenase: Enzymological Features, Type Systems, and Research Applications
梭菌蛋白酶的类型谱系、分子机制与科研应用综述
Clostridial Proteases: Classification, Molecular Mechanisms, and Research Applications
细胞核酸代谢与调控酶在基因表达、RNA稳态与免疫防御中的功能体系综述
A Functional Systems Review of Cellular Nucleic Acid Metabolism and Regulatory Enzymes in Gene Expression, RNA Homeostasis, and Immune Defense
组织单细胞制备的样本处理与质控策略
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
细胞样本保存体系对形态保持、抗凝稳定与检测结果的影响
Effects of Cell Sample Preservation Systems on Morphological Integrity, Anticoagulant Stability, and Analytical Results
组织消化与原代细胞分离中的广谱蛋白酶应用
Broad-Spectrum Protease Application in Tissue Digestion and Primary Cell Isolation
细胞凋亡检测中的TUNEL标记与结果判读
Cell Apoptosis Detection by TUNEL Labeling: Signal Interpretation and Result Evaluation
核酸酶在核酸样本处理、污染控制与分子实验质控中的应用
Application of Nucleases in Nucleic Acid Sample Processing, Contamination Control, and Molecular Experiment Quality Control
PDRN参与皮肤修复的机制:A2A受体相关信号、核苷酸补救与细胞外基质重塑
Mechanisms by Which PDRN Participates in Skin Repair: A2A Receptor-Related Signaling, Nucleotide Salvage, and Extracellular Matrix Remodeling
中性粒细胞与肿瘤:双重作用与靶向策略
Neutrophils and Cancer: Dual Roles and Targeting Strategies
此产品的引用文献
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