β-葡萄糖苷酶 (β-GC) 活性检测试剂盒 (对硝基苯酚, 微量法)

货号: G1521821
有货
级别和纯度: BioReagent ? 生物试剂级(BioReagent)—— 经测试适用于生命科学和分子生物学。适用于需要生物相容性的细胞培养、检测和生化工作。 标曲法 ?
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规格
库存
价格
数量
48T
G1521821-48T
期货 Stock Image
¥1,029.90
96T
G1521821-96T
现货 Stock Image
¥1,689.90
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为什么选择此级别

BioReagent,标曲法 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存,避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

β-葡萄糖苷酶(β-GC, EC3.2.1.21)广泛存在于动物、植物、微生物和培养细胞中,催化β-糖苷键水解,具有多方面生理作用:在纤维素的糖化作用中,β-GC负责进一步水解纤维素二糖和纤维素寡糖生成葡萄糖;β-GC 水解萜烯类香气前驱体,使糖苷键合态变成游离态,从而产生香味;β-GC能够水解植物体内野黑樱苷,释放HCN,从而防止昆虫取食。

本试剂盒检测原理是β-GC分解对-硝基苯-β-D-吡喃葡萄糖苷生成对-硝基苯酚,后者在400nm有最大吸收峰,通过测定吸光值升高速率来计算β-GC活性。


组分与储存:

组分货号组分名称 物理外观48T96T储存
G1521821A提取缓冲液液体 60 mL60 mL×22-8℃
G1521821B试剂一粉末1 vial1 vial-20℃. Store in the dark.
G1521821C试剂二液体12 mL24 mL2-8℃
G1521821D试剂三液体11 mL22 mL2-8℃
G1521821E标准品液体1 mL1 mL2-8℃. Store in the dark.
*请在实验前检查各组分的量。
*各组分在规格基础上额外提供10%的量用于进行标准曲线制作或预实验。

自备仪器与试剂:

自备资源类型资源名称备注
仪器酶标仪能测400nm处的吸光度
耗材96孔酶标板
普通透明板
试剂PBS、去离子水
清洗样本/配制试剂、稀释标准品用
其他匀浆器 (组织样本)、恒温箱、低温离心机、可调节式移液枪及枪头
检测量较大时合理使用多通道移液器有助于提高实验效率

注意事项:

1. 正式检测前,建议选择2-3个预期差异较大的样本进行预实验。

2. 对于组织样本及细胞样本等,可通过测定蛋白浓度来进行样本间结果均一化。推荐使用阿拉丁货号B665595 的BCA蛋白定量试剂盒。

3. 本试剂盒兼容分光光度计检测,请按照分光光度计检测要求按比例调节检测试剂配制量。

4. 建议实验者自行建立标准曲线以提高结果准确度。如未自行建立标准曲线,可参考结果展示部分的典型标准曲线公式进行结果计算。

5. 生化检测试剂普遍具有刺激性、生物毒性等,为了您的安全和健康,请在实验全程做好生物安全防护,穿戴实验服、口罩、手套、头套等安全护具,在通风橱、生物安全柜中进行实验。

6. 本产品仅供科学研究使用,不适用于临床诊断。


实验流程:

1. 试剂准备

1.1 试剂准备

试剂名称试剂准备注意事项
提取缓冲液即用型;使用前平衡到室温2-8℃保存
工作试剂一临用前配制; 向试剂一中加入去离子水配制, 48T 加入 9.6 mL 去离子水, 96T 加入 19.2 mL 去 离子水,充分溶解待用。未用完的试剂-20℃分装避光保存一个月,禁止反复冻融
试剂二即用型;使用前平衡到室温
2-8℃保存
试剂三即用型;使用前平衡到室温
2-8℃保存
标准品5μmol/mL标准液,即用型;使用前平衡到室温
2-8℃避光保存

1.2 标准品配制

使用5μmol/mL标准液,按照下表所示,进一步稀释标准品。每次实验都要做一次标准品检测,制作标曲;稀释后的标准品溶液不稳定,必须在4小时内使用。

序号标准品 (μL)去离子水 (μL)浓度 (nmol/mL)
120 μL 5 μmol/mL980100
2500 μL of 100 nmol/mL50050
3500 μL of 50 nmol/mL
50025
4500 μL of 25 nmol/mL
50012.5
5500 μL of 12.5 nmol/mL
5006.25
6500 μL of 6.25 nmol/mL
5003.125
7500 μL of 3.125 nmol/mL
5001.563
空白05000

2. 样本制备

注意:推荐使用新鲜样本,如果不立即进行实验,样本可在-80℃保存1个月。

2.1 动植物组织:称取约0.1g组织样本,加入1mL 提取缓冲液,冰浴匀浆,15000g,4℃离心10min,取上清,置冰上待测。

2.2 细胞:收集5×10⁶个细胞,用冷PBS清洗细胞,800g离心2min,弃上清液。加入1mL 提取缓冲液,冰浴超声波破碎细胞5min (功率20%或200W,超声3s,间隔7s,重复30次),15000g,4℃离心10min,取上清,置冰上待测。

2.3 血清(浆):直接测定。若溶液有浑浊则离心后取上清进行测定。

3. 实验步骤

3.1 酶标仪准备:预热30min以上,调节波长到400nm。

3.2 检测体系配制:在1.5m LEP管中按照如下方式操作,标准曲线和空白管只需做1-2次。

试剂测定管(μL)对照管(μL)标准管(μL)空白管(μL)
工作试剂一120000
试剂二15015000
去离子水012000
样本上清303000
标准品00700
去离子水00070
上清液707000
试剂三130130130130
加入样本上清后,充分混匀,放入37℃准确水浴30min后,立即放入95℃水浴5min(盖紧,以防止水分散失),冷却后充分混匀(以保证浓度不变),8000g,4℃,离心5min,取上清液(在96孔板中加入下列试剂)。

3.3 吸光度检测:充分混匀,室温静置2min后,于400nm处测定吸光值,分别记为A测定、A对照、A标准和A空白。


结果计算:

以下为您提供的推导过程计算公式和简洁计算公式,两者完全相等。

1. 数据处理

计算ΔA测定=A测定-A对照

ΔA标准=A标准-A空白

2. 标准曲线绘制

以标准品浓度为y轴,Δ标准为x轴,绘制标准曲线。将ΔA测定代入方程得到y值(nmol/mL)。

3. β-GC 活性的计算:

3.1 按样本蛋白浓度计算:

单位的定义:每mg组织蛋白每分钟产生1nmol对-硝基苯酚定义为一个酶活性单位。

β-GC (U/mg prot)=(y×V反总)÷(V样×Cpr)÷T=y÷Cpr÷3

3.2 按样本质量计算:

单位的定义:每g组织每分钟产生1nmol对-硝基苯酚定义为一个酶活性单位。

β-GC (U/g)=(y×V反总)÷(W×V样÷V样总)÷T=y÷W÷3

3.3 按细胞密度计算:

单位的定义:每10⁴个细菌每分钟产生1nmol对-硝基苯酚定义为一个酶活性单位。

β-GC (U/10⁴)=(y×V反总)÷(N×V样÷V样总)÷T=y÷N÷3

3.4 按样本体积计算:

单位的定义:每mL液体样本每分钟产生1nmol对-硝基苯酚定义为一个酶活性单位。

β-GC (U/mL)=(y×V反总)÷(V样÷V样总)÷T=y÷3


V反总:反应体系总体积,0.3mL;

V样:加入反应体系中样本体积,0.03mL;

V样总:加入提取缓冲液体积,1mL;

Cpr:样本蛋白质浓度,mg/mL;

W:样本质量,g;

N:细菌数量,以10⁴为单位(例如细菌数量为5×10⁶,N=500);

T:反应时间,30min。


结果展示:

典型标准曲线:

示例:取胎牛血清,按照测定步骤操作,用96孔板进行检测。测得ΔA测定=A测定-A对照=0.313-0.077=0.236,代入标准曲线,计算y=60.79。计算得:β-GC (U/mL)=(y×V反总)÷(V样÷V样总)÷T=y÷3=20.26 U/mL。


常见问题:

检测得到的样本ΔA测定过高或者过低怎么办?

答:如果样本ΔA测定>0.4,需用提取缓冲液 适当稀释或减少提取用样本量,重新进行测定。如果样本的ΔA测定<0.01,可适当提高样本量。

β‑Glucosidase (β‑GC, EC 3.2.1.21) is widely found in animals, plants, microorganisms and cultured cells. It catalyses the hydrolysis of β‑glycosidic bonds and exerts diverse physiological functions. During cellulose saccharification, β‑GC is responsible for further hydrolysing cellobiose and cello‑oligosaccharides to yield glucose. It hydrolyses terpene aroma precursors and converts glycosidically‑bound substances into the free‑state form to generate fragrance. Moreover, β‑GC can break down prunasin inside plant tissues and release hydrogen cyanide (HCN), which deters herbivorous insects from feeding.

The detection principle of this kit is as‑follows: β‑GC decomposes p‑nitrophenyl‑β‑D‑glucopyranoside to produce p‑nitrophenol. The latter possesses an absorption maximum at 400 nm. β‑GC activity is calculated by measuring the rising rate of absorbance.

Contents & Storage:

Component No.
Component Name
Appearance
48T96TStorage
G1521821AExtraction Buffer
Liquid  60 mL60 mL×22-8℃
G1521821BReagent Ⅰ
Powder
1 vial1 vial-20℃. Store in the dark.
G1521821CReagent Ⅱ
Liquid
12 mL24 mL2-8℃
G1521821DReagent Ⅲ
Liquid
11 mL22 mL2-8℃
G1521821EStandard
Liquid
1 mL1 mL2-8℃. Store in the dark.
*Check the volume of each component before the experiment.
*An extra 10 % quantity of each component is supplied on top of the specified‑size dosage for standard‑curve preparation or preliminary experiments.

Self-supplied Instruments and Reagents:

Type of Self-supplied Resources
Resource Name
Remarks
Instruments
Microplate reader
Capable of measuring absorbance at 400 nm
Consumables
96‑well microplate
Ordinary transparent plate
Reagents
PBS, deionized water
For sample washing, reagent preparation and standard‑product dilution.
Others
Homogenizer (for tissue samples), incubator, low‑temperature centrifuge, adjustable‑volume pipettes and pipette tips.
A multi‑channel pipette is recommended to boost experimental efficiency when processing a large number of test samples.

Precautions:

1. Before official detection, it is recommended to carry out a preliminary experiment using 2‑3 samples with large expected differences.

2. For tissue, cell and other specimens, normalize test results among samples via protein‑concentration determination. The BCA Protein Quantification Kit (Aladdin Cat. No. B665595) is recommended.

3. The kit supports spectrophotometer detection. Adjust the dosage of prepared detection reagents proportionally to meet spectrophotometer‑based testing requirements.

4. Researchers are suggested to establish a self‑made standard curve for higher‑precision data. When a custom‑made standard curve is unavailable, compute results with the typical standard‑curve formula provided in the result‑display section.

5. Most biochemical assay reagents are irritant and biologically hazardous. For your safety and wellbeing, maintain complete biosafety protection in the whole experimental process. Put on a lab coat, respirator, gloves, hair‑net and other protective equipment. Perform experimental operations in a fume hood or biosafety cabinet.

6. This product is for scientific‑research use only and shall not be adopted for clinical diagnosis.

Experimental Procedures:

1. Reagent Preparation

1.1 Reagent Preparation 

Reagent Name
Reagent Preparation
Notes
Extraction Buffer
Ready‑to‑use; equilibrate to room‑temperature prior to use.
Store at 2-8 °C
Working Reagent ⅠPrepare immediately before use. Reconstitute Reagent Ⅰ by adding deionized water: add 9.6 mL deionized water for the 48‑test kit and 19.2 mL deionized water for the 96‑test kit. Dissolve completely and set aside for use.Aliquot the leftover reagents and store them at −20 °C away from light for up to one‑month. Repeated freeze‑thaw cycles are prohibited.
Reagent Ⅱ
Ready‑to‑use; equilibrate to room‑temperature prior to use.
Store at 2-8 °C
Reagent Ⅲ
Ready‑to‑use; equilibrate to room‑temperature prior to use.
Store at 2-8 °C
Standard
5 μmol/mL standard solution, ready‑to‑use; equilibrate to room temperature prior to use.
2-8℃. Store in the dark.

1.2 Standard Preparation

Adopt the 5 μmol/mL standard solution to further dilute the standard substance in accordance with the table below. A standard‑curve assay shall be performed for every experiment. The diluted standard solutions are unstable and must be used within 4 hours.

No.
Standard (μL)
Deionized water (μL)Concentration (nmol/mL)
120 μL 5 μmol/mL980100
2500 μL of 100 nmol/mL50050
3500 μL of 50 nmol/mL
50025
4500 μL of 25 nmol/mL
50012.5
5500 μL of 12.5 nmol/mL
5006.25
6500 μL of 6.25 nmol/mL
5003.125
7500 μL of 3.125 nmol/mL
5001.563
Blank
05000

2. Sample Preparation

Note: Fresh samples are recommended. Samples may be stored at −80 °C for one month if the assay cannot be carried‑out immediately.

2.1 Animal and Plant Tissues

Weigh approximately 0.1 g of tissue sample, add 1 mL of Extraction Buffer, and homogenise on an ice bath. Centrifuge the mixture at 15 000 g and 4 °C for 10 min. Collect the supernatant and keep it on ice prior to measurement.

2.2 Cells

Harvest 5×10⁶ cells. Wash the cells with cold PBS and centrifuge at 800 g for 2 min, then discard the supernatant. Add 1 mL of Extraction Buffer and lyse the cells by ice‑bath ultrasonication for 5 min (power 20 % or 200 W; 3‑second sonication followed by a 7‑second interval, repeated 30‑times). Centrifuge at 15 000 g, 4 °C for 10 min. Collect the supernatant and preserve it on ice for subsequent testing.

2.3 Serum (Plasma)

Perform detection directly. Centrifuge turbid samples and take the supernatant for measurement.

3. Experimental Procedures

3.1 Microplate‑reader Preparation

Warm‑up the instrument for no less than 30 min and set the detection wavelength to 400 nm.

3.2 Preparation of Reaction System

Operate in 1.5‑mL EP tubes as described below. Prepare the standard‑curve tubes and blank tubes in one or two replicates only.


Reagent
Assay‑well (μL)Control‑well (μL)
Standard‑well (μL)
Blank‑well (μL)
WorkingReagentⅠ
120000
Reagent Ⅱ
15015000
Deionized water
012000
Sample supernatant
303000
Standard
00700
Deionized water
00070
Supernatant
707000
Reagent Ⅲ
130130130130
Thoroughly mix the liquid after adding the sample supernatant. Carry out precise water‑bath incubation at 37 °C for 30 min, then immediately heat the mixture in a 95 °C water bath for 5 min. Keep the tubes tightly capped to avoid water loss. Cool down and mix well to sustain a constant solute concentration. Centrifuge the mixture at 8000 g and 4 °C for 5 min, and collect the supernatant. Pipette the subsequent reagents into the 96‑well microplate.

3.3 Absorbance Measurement: Mix thoroughly, allow the mixture to stand at room temperature for 2 min, and then read the absorbance value at 400 nm. Record the readings as AAssay, AControl, AStandard and ABlank, respectively.


Calculation of Results

Both the derived computational formula and the simplified formula provided below are mathematically equivalent.

1. Data Processing

ΔAAssay=AAssay-AControl

ΔAStandard=AStandard-ABlank

2. Standard‑curve Plotting

Take the standard‑substance concentration as the y‑axis and ΔAStandard as the x‑axis to plot the standard curve. Substitute ΔAAssay into the regression equation to acquire the y‑value (unit: nmol/mL).

3. Calculation of β‑GC Activity

3.1 Calculation based on sample protein concentration

Definition of enzyme‑activity unit: One enzyme‑activity unit corresponds to 1 nmol p‑nitrophenol generated per milligram of tissue protein each minute.

β-GC (U/mg prot)=(y×Vtotal reaction)÷(Vsample×Cpr)÷T=y÷Cpr÷3

3.2 Calculation based on sample weight

Definition of enzyme‑activity unit: One enzyme‑activity unit corresponds to 1 nmol p‑nitrophenol generated per gram of tissue each minute.

β-GC (U/g)=(y×Vtotal reaction)÷(W×Vsample÷Vtotal sample)÷T=y÷W÷3

3.3 Calculation based on cell density

Definition of enzyme‑activity unit: One enzyme‑activity unit corresponds to 1 nmol p‑nitrophenol generated per 10⁴ bacteria each minute.

β-GC (U/mL)=(y×Vtotal reaction)÷(Vsample÷Vtotal sample)÷T=y÷3

3.4 Calculation based on sample volume

Definition of enzyme‑activity unit: One enzyme‑activity unit corresponds to 1 nmol p‑nitrophenol generated per millilitre of liquid sample each minute.

β-GC (U/mL)=(y×Vtotal reaction)÷(Vtotal sample÷Vtotal sample extract)÷T=y÷3

Vtotal reaction: total volume of the reaction system, 0.3 mL;

Vsample: volume of sample added into the reaction system, 0.03 mL;

Vtotal sample: volume of added Extraction Buffer, 1 mL;

Cpr: protein concentration of the sample, mg/mL;

W:sample weight, g;

N: bacterial count in units of 10⁴ (e.g. for a bacterial population of 5×10⁶, N = 500);

T: incubation reaction time, 30 min.

Results Presentation:

Typical Standard Curve:

Example: Take fetal bovine serum and perform the assay following the measurement‑procedure using a 96‑well microplate. The calculated ΔAAssay=AAssay-AControl=0.313-0.077=0.236, y=60.79。

β-GC (U/mL)=(y×Vtotal reaction)÷(Vsample‑loading÷Vtotal sample extract)÷T=y÷3=20.26 U/mL。


Frequently Asked Questions:

What should be done when the measured ΔAAssay of sample is excessively high or low?

Answer: If ΔAAssay >0.4, appropriately dilute the sample with Extraction Buffer or reduce the initial sample amount for extraction, then repeat the measurement. If ΔAAssay<0.01, increase the sample loading quantity appropriately.


规格

别名
β-葡萄糖苷酶 (β-GC) 活性检测试剂盒
英文别名
Beta-Glucosidase (β-GC) Activity Detection Kit Micromethod | β-glucosidase (β-GC) Activity Assay Kit
规格或纯度
BioReagent, 标曲法
英文名称
β‑Glucosidase (β‑GC) Activity Assay Kit (p‑Nitrophenol, Micro Method)
应用
酶活性检测
样品类型
动物组织, 植物组织, 血浆, 血清
检测方法
对硝基苯酚, 分光光度法
检测仪器
酶标仪
检测波长
400nm
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,-20°C储存
运输条件
超低温运输
稳定性与储存
各组分在相应的储存条件下保质期12个月。
组分与储存
组分货号组分名称物理外观48T96T储存
G1521821A提取缓冲液液体60 mL60 mL×22-8℃
G1521821B试剂一粉末1 vial1 vial-20℃. 避光
G1521821C试剂二液体12 mL24 mL2-8℃
G1521821D试剂三液体11 mL22 mL2-8℃
G1521821E
标准品
液体1 mL1 mL-20℃. 避光

注:

① 请在实验前检查各组分的量。

② 各组分在规格基础上额外提供10%的量用于进行标准曲线制作或预实验。

③ 整套试剂盒未拆封可于−20 ℃避光保存,自生产之日起有效期 12 个月。拆封后各组分稳定性不同:为避免反复冻融,组分 G1521821A、G1521821C、G1521821D、G1521821E 可转至 2‑8 ℃存放,有效期仍为 12 个月;组分 G1521821B 需继续冷冻保存。

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批号(Lot Number) 证书类型 货号
ZJ26F0838796 分析证书 G1521821
技术文档和文章
β-葡聚糖含量测定方法、样品前处理与质量控制
β-Glucan Content Determination Methods, Sample Pretreatment and Quality Control
细胞壁多糖降解酶的作用机制与实验应用:植物细胞壁、真菌细胞壁和细菌肽聚糖体系
Mechanisms and Experimental Applications of Cell Wall Polysaccharide-Degrading Enzymes: Plant Cell Walls, Fungal Cell Walls, and Bacterial Peptidoglycan Systems
D-甘露糖酶法制备技术:异构化转化、多糖水解与工艺控制
Enzymatic Preparation of D-Mannose: Isomerization Conversion, Polysaccharide Hydrolysis, and Process Control
纤维素降解关键酶的催化机制、功能分工与研究应用
Catalytic Mechanisms, Functional Division, and Research Applications of Key Enzymes in Cellulose Degradation
溶液计算器