计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
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EnzymoPure™,优级品 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 35 篇同行评审文献引用。
描述
胶原酶NB 1在色谱上得到高度纯化;因此,它具有很高的胶原蛋白分解活性。它基本上没有其他酶促活性,如梭菌蛋白酶,类胰蛋白酶活性和中性蛋白酶,以及内毒素。
规格
包含经色谱纯化的I类和II类胶原酶(1)。
基本上不含clostripain,胰蛋白酶样蛋白酶和中性蛋白酶。
小瓶中含有不少于2000 PZ U胶原酶。
活性(Wünsch):≥3.00 U / mg
内毒素:≤10.0 EU / mg(Ph。Eur。)
(Clostridiopeptidase A)
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
应用
胶原酶NB 1主要与中性蛋白酶NB结合使用,适用于从几种组织类型中分离细胞。
参考和定义
单位定义:
胶原酶:1 U在25°C(pH 7.1)下每分钟催化1 µmol 4-苯基偶氮苄氧基羰基-L-脯氨酰基-L-亮氨酰甘氨酰-L-脯氨酰基-D-精氨酸的水解。
内毒素:欧洲药典 (1个内毒素单位等于WHO批准的参考标准内毒素(RSE)的1个国际单位)。
References
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
Collagenase NB 1 is chromatographically highly purified; therefore it contains a very high collagenolytic activity. It is largely free from additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as endotoxins.
Specifications
Contains chromatographically highly purified class I and class II collagenase (1).
Largely free from clostripain, trypsin-like protease and neutral protease.
Vial contains not less than 2000 PZ U collagenase.
Activity (Wünsch): ≥ 3.00 U/mg
Endotoxin: ≤ 10.0 EU/mg (Ph. Eur.)
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Application
Collagenase NB 1 is, mostly in combination with Neutral Protease NB, suitable for cell isolation from several tissue types.
References and Definitions
Unit definition:
Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51.
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