Rabbit anti Human Carbonic anhydrase 2/CA2 Antibody, Recombinant, could be used for WB and so on. Application:
WB: 1/500-1/2000
IF/ICC: 1/200 Protein Function Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
规格
产品名称
Recombinant Carbonic anhydrase 2/CA2 Antibody
别名
碳酸酐酶2抗体 | 重组碳酸酐酶2/CA2抗体
英文别名
HEL-S-282 antibody | HEL-76 antibody | Epididymis secretory protein Li 282 antibody | epididymis luminal protein 76 antibody | Carbonic dehydratase antibody | Carbonic anhydrase II antibody | Carbonic anhydrase C, formerly antibody | CAII | CA2 | CA-IIEC
规格或纯度
重组, ExactAb™, 敲低验证, 已验证, 见COA
宿主种属
兔(Rabbit)
特异性
CA2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Carbonic anhydrase 2 (AA 200-260)
阳性对照
WB: Mouse brain, Rat brain, A431 and HEK293 lysates. IF/ICC: HeLa cells.
Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) - Western Blot All lanes: Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1.2 s
Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) - Western Blot All lanes: Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1.2 s
Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) - IF/ICC IF analysis of Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Carbonic anhydrase 2/CA2 Antibody (Ab092778) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).