计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
有货
为什么选择此级别
EnzymoPure™,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 35 篇同行评审文献引用。
概述
Collagenases from Clostridium histolyticum are proteolytic enzymes that cleave peptide bonds in the triple helical collagen molecules of human or animal tissue in situ. For this reason collagenases are widely used for isolation of various cell types by tissue dissociation.
Collagenase NB 5 Sterile Grade is sterile according to European Pharmacopoeia. Collagenase NB 5 Sterile Grade is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation.
Specification
Collagenase activity: ≥0.10 U/mg (PZ acc. to Wünsch)
Sterility: must comply
Application
Collagenase NB 5 Sterile Grade is suitable for dissociation of a broad variety of tissue types.
Storage conditions
Collagenase NB 5 Sterile Grade is available as a lyophilized powder.
It should be stored at +2 to +8℃ in a dry environment. Under these conditions the product is stable until the minimum shelf life stated on the certificate of analysis if repeated opening and closing of the vial is avoided. For storage of solutions please refer to “Stock solution”.
Instructions for use:
General
Collagenase NB 5 Sterile Grade is suitable for isolation of a broad variety of cells from human or animal tissues. Tissue types include adipose tissue, cartilage, skin, placenta, and umbilical cord tissue. It can also be applied in cell culture for passaging, e.g. of embryonic stem cells.
Tissue dissociation
Recommended starting concentrations for selected applications:
Adipose tissue (human, rodent): 0.2 – 0.3 PZ U/ml
Cartilage (human, rodent): 0.3 – 0.4 PZ U/ml
In general, the appropriate collagenase concentration depends on tissue type and origin as well as on the isolation procedure.
Collagenase activity is at an optimum at 37℃ and pH 7.4.
Stock solution
Collagenase NB 5 Sterile Grade dissolves at a concentration of up to 150 mg/ml in all buffers which are commonly used for cell isolation. The enzyme solution must be constantly stored on ice.
Since collagenase and some of the secondary proteases depend on calcium, it is recommended to use a buffer with ≥ 2 mM Ca²⁺. Absolutely no calcium chelating agents (e.g. EDTA) should be present at all.
Reconstituted Collagenase NB 5 Sterile Grade can be aliquoted and stored at -20 °C. Aliquots are stable for 1 year if repeated freezing and thawing is avoided.
Collagenase NB 5 Sterile Grade is sterile according to Ph. Eur. Therefore, 0.22 µm filtration is not necessary if sterile equipment and buffers are used. If 0.22 µm filtration is required, filters with low protein-binding properties (e.g. cellulose acetate, PVDF, or PES) are recommended.
Working solution
To prepare a working solution, the stock solution is diluted with buffer to achieve the required collagenase concentration. The working solution must be constantly stored on ice until use.
Inactivation and inhibitors
The dissociation process can be reduced, e.g. by cooling down or dilution of the enzyme solution.
Collagenase is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine or chelating agents like EDTA.
Important note
Collagenase NB 5 Sterile Grade is intended for research use only.
规格
产品名称
胶原酶 NB 5 来源于溶组织梭菌
英文别名
Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene | microbial c
规格或纯度
EnzymoPure™, 无菌, ≥0.10 U/mg
生化机理
Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen. Catalytic activity: Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.
生物活性
≥0.10 U/mg
种属
溶组织梭菌(Clostridium histolyticum)
来源
天然
预测分子量
70–120 kDa
CAS编号和信息
9001-12-1
酶学委员会编号
3.4.24.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥0.10 U/mg
复溶
建议通过将胶原酶 NB 溶解在适当体积的缓冲液（例如 HBSS，PBS，Tris 或 Ringer 溶液）中制成储备溶液。 配制好的酶应进行无菌过滤（如果不是无菌的话），分装并保存在-20℃下。 我们的稳定性研究表明，如果避免反复冷冻和解冻，该酶在这些条件下可以维持稳定一年。
储存条件
2-8°C储存
运输条件
低温运输
稳定性与储存
长期储存2-8℃（大于1年）；复溶后建议分装。
单位定义
The hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25℃, pH 7.1.
技术文档
高级数据
关联靶点（人）
HT-29
(80576 活性数据)
MCF7
(126967 活性数据)
SK-MEL-5
(47095 活性数据)
A549
(127892 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0826196 |
分析证书 | C279009 |
| ZJ25F0826197 |
分析证书 | C279009 |
技术文档和文章
多种组织单细胞解离酶的应用与优化
目录： 技术文章
常见组织解离酶的原理与选择策略
目录： 技术文章
Photo-Crosslinking and Patterning of PEG-Based Hydrogels (2D/3D): Principles, Method Comparisons, and Product Selection Guidelines
目录： 技术文章
Cholesterol Esterase: Mechanistic Insights and Application Frameworks Driven by Interfacial Hydrolysis
目录： 技术文章
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
目录： 技术文章
Designing Hydrogel Scaffolds as Programmable Cell Microenvironments: From Click-Chemistry Functionalization to 3D Cell Culture Experimental Design
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
How Palmitoyl Pentapeptide-4 Acts on the Skin: From the KTTKS Sequence and Skin Delivery to Collagen Matrix Responses
目录： 技术文章
How Does Collagen Tripeptide Act on the Skin: Stratum Corneum Delivery, Matrix Protection, and Regulation of Collagen Homeostasis
目录： 技术文章
What Determines Collagen Function: From Triple-Helix Formation to Hierarchical Assembly, Crosslink Maturation, and Dynamic Remodeling
目录： 技术文章
What Is “Plant Collagen”? Distinguishing Ingredient Types, Structural Differences, Production Methods, and Skincare Effects
目录： 技术文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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