DNA酶I

CAS: 9003-98-9 货号: D128590 EC号: 232-667-0
有货
级别和纯度: 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 分子生物学级 ? 适用于分子生物学 —— 不含降解 DNA/RNA 的核酸酶和污染物。适用于克隆、PCR 和需要洁净试剂的核酸工作。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 无蛋白酶 ? 无蛋白酶 —— 经验证无蛋白酶活性,保护蛋白质和酶。适用于须避免蛋白水解的蛋白制备和检测。 无RNA酶 ? 无 RNase —— 经验证无 RNase 活性,保护易损 RNA。适用于 RNA 提取、RT-PCR 及任何对 RNA 敏感的流程。 来源于牛胰腺; ≥2,000 Kunitz units/ml
Accession #
P00639
生物活性
≥2,000 Kunitz units/ml
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规格
库存
价格
数量
100U
D128590-100UN
现货 Stock Image
¥587.90
500U
D128590-500UN
现货 Stock Image
¥2,059.90
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为什么选择此级别

EnzymoPure™,分子生物学级,天然,无RNA酶,无蛋白酶 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 45 篇同行评审文献引用。

概述


Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.

Uses: This product is especially designed for Molecular Biology applications and contain the lowest levels of RNase and protease activity. They are both suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, plasmid construction, and RNA polymerase synthesis of RNA probes and RT-PCR.

Unit Definition
1 Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25 °C when acting upon highly polymerized DNA at pH 5.0.
Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Aladdin.
As measured at Aladdin, one Kunitz unit digests 1 µg of lambda DNA in 10 minutes at 37 °C in 50 mM Tris, 1 mM Mg²⁺, 1 mM Ca²⁺, pH 7.8 in a 50 µl reaction.
Correlation of digestion units with Kunitz units is different for other DNA and buffer systems.

Characteristics of Deoxyribonuclease I
Protein Accession Number: P00639
CATH Classification
Class: Alpha Beta
Architecture: 4-Layer Sandwich
Topology: Deoxyribonuclease I; Chain A
Molecular Weight: 29.1 kDa (Theoretical)
Optimal pH: 7.8
Isoelectric point: 5.08 (Theoretical)
Extinction Coefficient: 36,750 cm⁻¹·M⁻¹;E₁%,₂₈₀ = 11.1
Active Site Residues: Tyrosine (Y87), Glutamate (E100), Histidine (H156)
Activators: Bivalent metal ions,

Inhibitors: 2-mercaptoethanol, 2-nitro-5-thiocyanobenzoic acid, Actin, Alfatoxin B2a, G2, G2a, and M1 (non-competitive), EGTA and EDTA, Sodium dodecyl sulfate, Calf spleen inhibitor protein, Carbodiimide and cholesterol sulfate, Iodoacetate

规格

产品名称
DNA酶I
别名
脱氧核糖核酸酶 I
规格或纯度
天然,分子生物学级,EnzymoPure™,无蛋白酶,无RNA酶,来源于牛胰腺; ≥2,000 Kunitz units/ml
生化机理
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity). Catalytic activity: Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products. Cofactor: Ca²⁺. Mg²⁺. Note: Divalent metal cations. Prefers Ca²⁺ or Mg²⁺. Post-translational: The only differences between the A and B forms and the C and D forms are in the compositions of the carbohydrate bound to Asn-40.
生物活性
≥2,000 Kunitz units/ml
种属
牛(Bovine)
来源
天然
预测分子量
29.1 kDa
CAS编号和信息
9003-98-9
酶学委员会编号
3.1.21.1
分子类型
酶
储存与运输
物理形态
液体
储存缓冲液
50% Glycerol, 1 mM CaCl₂.
浓度
来源于牛胰腺; ≥2,000 Kunitz units/ml
储存条件
-20°C储存
运输条件
超低温运输
单位定义
1 Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25 °C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Aladdin.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到6个结果

批号(Lot Number) 证书类型 货号
E1522102 分析证书 D128590
F2604642 分析证书 D128590
F2617611 分析证书 D128590
K2211210 分析证书 D128590
F2519041 分析证书 D128590
K2211224 分析证书 D128590
技术文档和文章
CRISPR-Cas9和靶向基因组编辑
流式实验样品制备实验指南
Experimental Guide of Sample Preparation for Flow Cytometry
CUT&RUN-seq实验方案
RNA提取和反转录实验方案
RNA Extraction and Reverse Transcription Protocol
如何选择血浆游离RNA提取试剂
How to Choose Plasma Cell-Free RNA (cfRNA) Extraction Reagents
多种组织单细胞解离酶的应用与优化
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
常见组织解离酶的原理与选择策略
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
His-Tag蛋白纯化时常见问题及解决方法
His-Tag Protein Purification: Common Troubleshooting Questions and Practical Solutions
Proteinase K常见问题
Proteinase K: Frequently Asked Questions
植物组学技术全景:基因组—转录组—蛋白质组—代谢组的流程框架与多组学整合应用
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
RNA提取技术:方法体系、操作流程与质量控制要点
RNA Extraction Technologies: Method Systems, Operational Workflows, and Key Quality-Control Considerations
脱氧核糖核酸酶(DNase):DNA水解切割特性、方法学价值与多场景应用
Deoxyribonuclease (DNase): DNA Hydrolytic Cleavage Properties, Methodological Value, and Multi-Context Applications
分散酶(Dispase):基底膜松解型组织解离蛋白酶的性质、制备与应用体系
白介素29(IL-29/IFN-λ1):分子特征、信号网络与科研应用体系
Interleukin-29 (IL-29/IFN-λ1): Molecular Features, Signaling Networks, and a Systematic Framework for Research Applications
二苯胺的理化特性、分析应用及其与DNA显色反应综述
Physicochemical Properties of Diphenylamine, Analytical Applications, and a Review of Its DNA Colorimetric Reaction
胶原酶(Collagenase)的酶学特征、类型体系与科研应用综述
Review of Collagenase: Enzymological Features, Type Systems, and Research Applications
梭菌蛋白酶的类型谱系、分子机制与科研应用综述
Clostridial Proteases: Classification, Molecular Mechanisms, and Research Applications
组织单细胞制备的样本处理与质控策略
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
细胞样本保存体系对形态保持、抗凝稳定与检测结果的影响
Effects of Cell Sample Preservation Systems on Morphological Integrity, Anticoagulant Stability, and Analytical Results
细胞凋亡检测中的TUNEL标记与结果判读
Cell Apoptosis Detection by TUNEL Labeling: Signal Interpretation and Result Evaluation
CRISPR/Cas9 and targeted genome editing
CUT&RUN-seq protocol
PDRN参与皮肤修复的机制:A2A受体相关信号、核苷酸补救与细胞外基质重塑
Mechanisms by Which PDRN Participates in Skin Repair: A2A Receptor-Related Signaling, Nucleotide Salvage, and Extracellular Matrix Remodeling
中性粒细胞与肿瘤:双重作用与靶向策略
Neutrophils and Cancer: Dual Roles and Targeting Strategies
此产品的引用文献
溶液计算器