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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
ATP 广泛存在于动物、植物、微生物和培养细胞中，是细胞内的生物能量通货，在细胞的各种生理和病理过程中发挥重要作用。能荷是描述细胞能量代谢状态的主要参数。通过测定机体ATP 含量并计算能荷，对机体能量代谢状态分析具有重要意义。 本试剂盒检测原理是肌酸激酶催化肌酸和 ATP 反应生成磷酸肌酸。在酸性条件下，磷酸肌酸分解为肌酸和无机磷。在还原剂的作用下，无机磷与钼酸铵反应产生深蓝色钼蓝复合物，测定其在700 nm处的吸光度值即可计算样本中 ATP 的含量。
Adenosine triphosphate (ATP) is widely present in animals, plants, microorganisms, and cultured cells. It serves as the universal biological energy currency within cells and plays a vital role in various physiological and pathological processes. Energy charge is a key parameter describing the state of cellular energy metabolism. Determining the ATP content in an organism and calculating its energy charge is of great significance for analyzing the organism's energy metabolic status. The detection principle of this kit is as follows: creatine kinase catalyzes the reaction of creatine and ATP to generate phosphocreatine. Under acidic conditions, phosphocreatine decomposes into creatine and inorganic phosphate. In the presence of a reducing agent, the inorganic phosphate reacts with ammonium molybdate to produce a dark blue molybdenum blue complex. The ATP content in the sample can be calculated by measuring the absorbance of this complex at 700 nm.
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