Mouse anti human RPA32/RPA2 Antibody, Monoclonal (1591CT183.68.6), could be used for WB, IHC and so on.
Application:
WB: 1/4000
IHC：1/100~1/500
Protein Function:
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
RPA32/RPA2 Mouse mAb (Ab125728) - Western Blot
All lanes: RPA32/RPA2 Mouse mAb (Ab125728) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 31 kDa
Exposure time: 50.0 s
RPA32/RPA2 Mouse mAb (Ab125728) - IHC
RPA32/RPA2 Mouse mAb (Ab125728) staining RFA2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
1/100-1/500
1/4000
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0102589 |
分析证书 | Ab125728 |
| ZJ24F0102588 |
分析证书 | Ab125728 |
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