Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.

Crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized.

规格

产品名称

α-糜蛋白酶来源于牛胰腺

别名

α-胰凝乳蛋白酶

英文别名

Chymotrypsin Substrate I, Colorimetric | 4-[[2-[[2-[[1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-2-oxoethyl]amino]-2-oxoethyl]amino]-4-oxobutanoic acid | CHYMOTRYPSIN | a-Chymotrypsin

规格或纯度

EnzymoPure™, ≥35 units/mg protein

生化机理

一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。 α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.

CAS编号和信息

9004-07-3

分子类型

酶

储存与运输

浓度

≥35 units/mg protein

储存条件

2-8°C储存

运输条件

低温运输

单位定义

One Unit hydrolyzes one micromole of benzoyl-L-tyrosine ethyl ester per minute at 25°C, pH 7.8 in the presence of calcium. An activity of 45 units per mg using the above definition, is the equivalent of 10,000 optical density or 1330 N.F. units per mg usi

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批号(Lot Number)	证书类型	货号
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蛋白样品制备流程

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Protein sample preparation process

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Protein sample preparation

蛋白质分子量标准

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Protein Molecular Weight Standards

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protein Protein Molecular Weight Standards

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A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications

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科研常用蛋白酶的基础介绍与典型应用

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Commonly Used Proteases in Research: Fundamentals and Representative Applications

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Proteomics proteases peptide-bond hydrolysis substrate specificity limited proteolysis antibody fragmentation deproteinization industrial enzyme preparations

糜蛋白酶（Chymotrypsin）的酶学特性、催化机制与科研应用综述

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肽图丝氨酸蛋白酶底物特异性催化三联体糜蛋白酶 α-糜蛋白酶氧阴离子孔酰酶中间体限制性蛋白水解方法学质控

Review of Chymotrypsin: Enzymological Properties, Catalytic Mechanism, and Research Applications

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Peptide Mapping Serine protease substrate specificity limited proteolysis catalytic triad Chymotrypsin Alpha-Chymotrypsin Oxyanion Hole Acyl-Enzyme Intermediate Methodological Quality Control

IHC实验中低pH、高pH及酶法抗原修复体系的应用比较

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Comparison of Low-pH, High-pH, and Enzymatic Antigen Retrieval Systems in IHC

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Advanced enzyme analysis technology

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内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用

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Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research

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生物模拟肽的皮肤作用逻辑：序列与构象、皮肤递送、稳定性及靶点匹配

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脂质体生物模拟肽序列仿生信号肽角质层递送脂质化修饰蛋白酶稳定性穿胞肽细胞靶点匹配细胞外基质调节

The Logic of Biomimetic Peptide Action in Skin: Sequence and Conformation, Skin Delivery, Stability, and Target Matching

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liposomes Cell-Penetrating Peptides biomimetic peptides sequence mimicry signaling peptides stratum corneum delivery lipidation proteolytic stability cellular target matching extracellular matrix regulation

丝氨酸内切蛋白酶的催化机制、分类特征与实验应用

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Catalytic Mechanisms, Classification Characteristics, and Experimental Applications of Serine Endopeptidases

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