计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 35 篇同行评审文献引用。
概述
来自溶组织梭菌（ Clostridium histolyticum ）的胶原酶被用于以下应用：足细胞分离用于酶催化法的对比用于免疫细胞化学的组织制备睾丸精子提取制备单细胞悬浮液免疫荧光法本品适用于人类肿瘤、小鼠肾脏、人类成人和胎儿的脑、肺和许多其他上皮组织的解离。 它还被证明适用于肝脏和肾脏灌注研究、胰腺消化、非实质性大鼠肝细胞的分离、以及肝细胞制备。 胶原酶也已用于制备动脉组织，以对晚期糖基化终产物进行研究。 该酶用于（浓度约为1mg/ml）对肝细胞的释放进行检测。消化浓度范围为0.1至5mg/mL。适用于制备用于测序的单细胞悬液。应用：
胶原酶是一种蛋白酶，可切割三螺旋结构的蛋白，即胶原蛋白。组织胶原酶有三种类型，它们属于基质金属蛋白酶（MMP）家族。来自溶组织梭菌（ Clostridium histolyticum ）的胶原酶具有非常强的活性，它在低至4-10℃的温度下可从两端消化胶原蛋白。 粗制胶原酶混合物含有两种主要类型的酶，即胶原酶和梭菌蛋白酶。
Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10°C. Crude collagenase mixtures contain two major enzyme types namely, collagenase and clostripain.
Application:Collagenase from Clostridium histolyticum is used for the following applications:
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1mg/mL. Concentrations for digestion range from 0.1 to 5mg/mL.
Suitable for use in preparation of single cell suspension for sequencing.
- Sertoli cell isolation
- Used in the comparison of enzymatic methods
- Used during tissue preparations for immunocytochemistry
- Testicular sperm extraction
- Preparation of single cell suspensions
- Immunofluorescence
This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain, lung and many other epithelia tissues. It has also been shown to be effective in liver and kidney perfusion studies, digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation. Collagenase has also been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products. This enzyme has been tested for the release of heptatocytes at a concentration of approximately 1mg/mL. Concentrations for digestion range from 0.1 to 5mg/mL.
Suitable for use in preparation of single cell suspension for sequencing.
规格
产品名称
胶原酶 来源于溶组织梭菌
规格或纯度
lyophilized powder,≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid
生化机理
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。胶原酶产品是一种由溶组织梭菌( C.histolyticum)分泌的酶的混合物，分泌酶中10-18种化合物成分的相对比例在不同产品中有所差异。 主要成分是两种胶原酶、梭菌蛋白酶和中性蛋白酶。 这些酶的协同作用可降解胶原蛋白和其他细胞内物质。胶原酶和中性蛋白酶的作用是细胞从组织有效释放所必需的。 各种类型的胶原蛋白是胶原酶的天然底物。
CAS编号和信息
9001-12-1
酶学委员会编号
3.4.24.3(BRENDA,IUBMB)
分子类型
小分子
储存与运输
物理形态
固体
浓度
lyophilized powder,≥125 CDU/mg solid (CDU = collagen digestion units), 0.5-5.0 FALGPA units/mg solid
储存条件
-20°C储存
运输条件
超低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
多种组织单细胞解离酶的应用与优化
目录： 技术文章
常见组织解离酶的原理与选择策略
目录： 技术文章
Photo-Crosslinking and Patterning of PEG-Based Hydrogels (2D/3D): Principles, Method Comparisons, and Product Selection Guidelines
目录： 技术文章
Cholesterol Esterase: Mechanistic Insights and Application Frameworks Driven by Interfacial Hydrolysis
目录： 技术文章
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
目录： 技术文章
Designing Hydrogel Scaffolds as Programmable Cell Microenvironments: From Click-Chemistry Functionalization to 3D Cell Culture Experimental Design
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
How Palmitoyl Pentapeptide-4 Acts on the Skin: From the KTTKS Sequence and Skin Delivery to Collagen Matrix Responses
目录： 技术文章
How Does Collagen Tripeptide Act on the Skin: Stratum Corneum Delivery, Matrix Protection, and Regulation of Collagen Homeostasis
目录： 技术文章
What Determines Collagen Function: From Triple-Helix Formation to Hierarchical Assembly, Crosslink Maturation, and Dynamic Remodeling
目录： 技术文章
What Is “Plant Collagen”? Distinguishing Ingredient Types, Structural Differences, Production Methods, and Skincare Effects
目录： 技术文章
此产品的引用文献
溶液计算器
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