胶原蛋白酶来源于溶组织梭菌(无动物源性,B)

Name: 胶原蛋白酶 来源于溶组织梭菌(无动物源性,B)
SKU: C128711
Availability: InStock

CAS: 9001-12-1 货号: C128711 EC号: 232-582-9 PubChem CID: 44384985

35篇引用文献 COA

有货

级别和纯度: 无动物源 ? 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? ≥300 units/mg dry weight

Accession #

Q46085,Q9X721

生物活性

≥300 units/mg dry weight

规格

库存

价格

数量

50mg

C128711-50mg

现货

¥649.90

250mg

C128711-250mg

现货

¥2,499.90

C128711-1g

现货

¥6,999.90

C128711-5g

现货

¥24,999.90

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概述规格储存与运输技术文档高级数据关联靶点（人）作用机制质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,无动物源,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 35 篇同行评审文献引用。

概述

Collagenase from Clostridium histolyticum(Animal Origin Free,B) is prepared from cultures grown in medium devoid of animal based components to contain higher collagenase and caseinase activities than C128710.

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca²⁺ is required for enzyme activity.

Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.

• Type 1 crude collagenase has the original balance of collagenase, caseinase, clostripain and tryptic activities.

• Type 2 contains higher relative levels of protease activity, particularly clostripain.

• Type 3 contains lowest levels of secondary proteases.

• Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.

The collagenase assay is a modification of Mandl wherein collagenase is incubated for five hours with native collagen and the extent of collagen breakdown is determined using the Moore and Stein, JBC, 176, 367, (1948) colorimetric ninhydrin method. Amino acids released are expressed as micromoles leucine per milligram collagenase.

Uses: Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (Code: C128695); the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc. For best results, the precise mixture of proteolytic activities must be tailored to the tissue to be dissociated. Correlations between type and effectiveness with different tissues have been good, but not perfect, and may be dependent partly on parameters of use and objectives as well as lot-to-lot variations.

规格

产品名称

胶原蛋白酶来源于溶组织梭菌(无动物源性,B)

别名

梭菌肽酶 A | 梭菌肽酶 A | 胶原酶 (B型, 无动物源)

英文别名

Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene | microbial c

规格或纯度

无动物源, 生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, ≥300 units/mg dry weight

生物活性

≥300 units/mg dry weight

种属

溶组织梭菌(Clostridium histolyticum)

来源

天然

CAS编号和信息

9001-12-1

酶学委员会编号

3.4.24.3

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥300 units/mg dry weight

储存条件

2-8°C储存

运输条件

低温运输

单位定义

One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

关联靶点（人）

HT-29 (80576 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SK-MEL-5 (47095 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

A549 (127892 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到10个结果

批号(Lot Number)	证书类型	货号
L2205709	分析证书	C128711
L1805086	分析证书	C128711
L1805085	分析证书	C128711
D2610629	分析证书	C128711
G2631055	分析证书	C128711
G2331013	分析证书	C128711
G2331029	分析证书	C128711
G2623029	分析证书	C128711
G2623031	分析证书	C128711
I2502070	分析证书	C128711

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目录：技术文章

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Advanced enzyme analysis technology

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蛋白样品制备流程

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巨噬细胞活化

目录：技术文章

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Macrophage Activation

目录：技术文章

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多种组织单细胞解离酶的应用与优化

目录：技术文章

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Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes

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Photo-Crosslinking and Patterning of PEG-Based Hydrogels (2D/3D): Principles, Method Comparisons, and Product Selection Guidelines

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Polyethylene Glycol (PEG) PEG Hydrogels

科研常用蛋白酶的基础介绍与典型应用

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Proteomics proteases peptide-bond hydrolysis substrate specificity limited proteolysis antibody fragmentation deproteinization industrial enzyme preparations

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生物催化胆固醇酯酶胆固醇酯水解界面催化胆盐激活总胆固醇检测脂质组学脂蛋白酶固定化

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Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies

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cell viability single-cell preparation tissue processing sample quality control enzymatic digestion doublets declumping dead cell removal single-cell transcriptomics flow sorting

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