计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 51 篇同行评审文献引用。
L-谷氨酸 (L-Glutamic acid)分子量为147.13,分子式为C₅H₉NO₄,CAS号为56-86-0,是一种酸性氨基酸。分子内含两个羧基,化学名称为α-氨基戊二酸。谷氨酸是里索逊1856年发现的,为无色晶体,有鲜味,微溶于冷水,易溶于热水,可溶于盐酸溶液,等电点3.22。谷氨酸大量存在于谷类蛋白质中,动物脑中含量也较多,在生物体内的蛋白质代谢过程中占重要地位,参与动物、植物和微生物中的许多重要化学反应。谷氨酸是生产味精、香料的原料,亦可用作哺乳动物细胞培养中培养基的添加剂,用途极为广泛。
L-谷氨酸溶液 (0.2 mol/L)主要由L-谷氨酸 (约2.9%)、去离子水组成,经过滤除菌处理。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤 (仅供参考):
1、本产品从低温冰箱拿出后,可先置于室温自然解冻后,仍有白色不溶物属正常现象,可置于60-65℃水浴中助溶,45-60min即可完全复溶,呈完全透明状态。水浴温度不可超过75℃,否则瓶子可能变形。
2、按实验具体要求操作。一般按培养基: L-谷氨酸溶液=100: 1使用。
注意事项:
1、注意避免反复冻融,并且注意无菌操作。首次溶解后建议分装小份,低温保存。
2、L-谷氨酸是白色结晶性粉末、几乎无臭,224℃-225℃分解,饱和水溶液的pH值约为3.2。难溶于水,实际不溶于乙醇和乙醚、极易溶于甲酸。水中溶解度 (g/100g水)为0.72 (20℃),1.51 (40℃),3.17 (60℃),6.66 (80℃)。
3、试剂开封后请尽快使用,以防影响后续实验效果。
4、为了您的安全和健康,请穿实验服并戴一次性手套操作。
L-Glutamic acid has a molecular weight of 147.13, a molecular formula of C₅H₉NO₄, and a CAS number of 56-86-0. It is a type of acidic amino acid, containing two carboxyl groups in its molecule, and its chemical name is α-aminoglutaric acid. First discovered by Ritthausen in 1856, glutamic acid appears as colorless crystals with a savory taste. It is slightly soluble in cold water, readily soluble in hot water, and soluble in hydrochloric acid solution, with an isoelectric point of 3.22.
Glutamic acid is abundant in cereal proteins and also present in high concentrations in animal brains. It plays a crucial role in protein metabolism in organisms and participates in numerous important chemical reactions in animals, plants, and microorganisms. It serves as a raw material for producing monosodium glutamate (MSG) and fragrances, and can also be used as an additive in culture media for mammalian cell culture, boasting extremely wide applications.
The L-Glutamic Acid Solution (0.2 mol/L) is mainly composed of L-glutamic acid (approximately 2.9%) and deionized water, and is sterilized by filtration. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only)
1. After removing the product from the low-temperature refrigerator, allow it to thaw naturally at room temperature first. The presence of white insoluble substances is a normal phenomenon. The solution can be placed in a water bath at 60-65°C to assist dissolution; it will fully redissolve into a completely transparent state within 45-60 minutes. Do not exceed 75°C in the water bath, as this may cause deformation of the bottle.
2. Operate according to the specific requirements of the experiment. Generally, it is used at a ratio of culture medium: L-Glutamic Acid Solution=100: 1.
Precautions
1. Avoid repeated freezing and thawing, and ensure aseptic operation during use. After the first dissolution, it is recommended to aliquot the solution into small portions and store them at low temperature.
2. L-Glutamic acid is a white crystalline powder with almost no odor. It decomposes at 224-225°C, and the pH value of its saturated aqueous solution is approximately 3.2. It is sparingly soluble in water, practically insoluble in ethanol and ether, and highly soluble in formic acid. Its solubility in water (g/100g water) is as follows: 0.72 (20°C), 1.51 (40°C), 3.17 (60°C), and 6.66 (80°C).
3. Please use the reagent as soon as possible after opening to prevent any adverse effects on subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
H314: 造成严重的皮肤灼伤和眼睛损伤
H318: 造成严重的眼睛损伤
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾
P305+P351+P338: 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。
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