辣根过氧化物酶(Horseradish Peroxidase, HRP)广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,是临床检验试剂中的常用酶。该产品不但广泛用于多个生化检测项目,也广泛运用于免疫类(ELISA)试剂盒。过氧化物酶作为多个试剂盒显色体系的关键成分,对试剂盒的质量有重要影响。
产品特性
1. 辣根过氧化物酶比活性高,稳定,分子量小,纯酶容易制备,所以最常用。
2. HRP 的辅基和酶蛋白最大吸收光谱分别为 403 nm 和 275 nm,一般以 OD₄₀₃ nm / OD₂₇₅ nm 的比值 RZ(德文 Reinheit Zahl)表示酶的纯度。
APPLICATION 应用
1. 辣根过氧化物酶(RZ3.0),主要应用于免疫学,是高纯度的过氧化物酶。采用特殊色谱纯化技术以除去会影响免疫学反应的同工酶 B;
2. 辣根过氧化物酶(RZ2.0/RZ2.5),主要应用于临床化学,也有将这个规格的产品应用于免疫学研究的;
3. 辣根过氧化物酶(RZ1.5),主要应用于血糖试纸和尿液分析试纸;
4. 辣根过氧化物酶(RZ0.4),主要应用于尿液分析试纸。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。
Horseradish Peroxidase (HRP) is widely distributed in the plant kingdom, with high content in horseradish. It is a glycoprotein formed by the combination of colorless enzyme proteins and brown iron porphyrins, and is a commonly used enzyme in clinical laboratory reagents. This product is not only widely used in multiple biochemical testing projects, but also widely used in immunological (ELISA) test kits. Peroxidase, as a key component of the color display system of multiple test kits, has a significant impact on the quality of the test kits.
PROPERTIES
1. Horseradish peroxidase is most commonly used due to its high specific activity, stability, small molecular weight, and easy preparation of pure enzymes.
2. The maximum absorption spectra of HRP cofactors and enzyme proteins are 403 nm and 275 nm, respectively. Generally, the purity of the enzyme is represented by the ratio of OD₄₀₃ nm/OD₂₇₅ nm RZ (German Reinheit Zahl).
APPLICATION
1. Horseradish peroxidase (RZ3.0), mainly used in immunology, is a high-purity peroxidase. Using special chromatographic purification techniques to remove isoenzyme B that can affect immunological reactions;
2. Horseradish peroxidase (RZ2.0/RZ2.5) is mainly used in clinical chemistry, and there are also products of this specification used in immunological research;
3. Horseradish Peroxidase (RZ1.5), mainly used in blood glucose test strips and urine analysis test strips;
4. Horseradish Peroxidase (RZ0.4) is mainly used in urine analysis test strips.
ATTENTION
For your safety and health, please wear a lab coat and disposable gloves.