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一般描述
聚乙二醇(PEG)是环氧乙烷和水通过缩合反应产生的聚合物。
应用
推荐用于需要50%PEG的正常融合方案。聚乙二醇溶液已用于以下细胞的杂交:
1,辐照骆驼成纤维细胞与A23仓鼠细胞
2,小鼠脾细胞与Sp2/0-Ag 14小鼠骨髓瘤细胞
3,兔脾细胞和细胞系240E-1
聚乙二醇可用作融合剂,以获得生产单克隆抗体的杂交瘤细胞。诱导细胞杂交。
其他说明
含50%(w/v)聚乙二醇(平均分子量1450)的DPBS溶液,不含钙。
重悬
即用型溶液。如果需要浓度较低的溶液,可使用不含钙的无菌DPBS(D5773)稀释。暴露于较低温度后可能会出现一些沉淀物,但随着溶液温度升高,沉淀应该会消失。 如有需要,可以冷冻溶液,但应该先分装再冷冻,以避免反复冻融。
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Application
Polyethylene glycol solution has been used in the hybridization of the following:
1, irradiated camel fibroblast cells with A23 hamster cells
2, mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
3, rabbit splenocytes and cell line 240E-1
Recommended for use in a normal fusion protocol requiring 50% PEG.PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Reconstitution
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.