DNase I 在大多数细胞和组织中都能发现的一种核酸内切酶，偏好切割邻近嘧啶的磷酸二酯键，产生 5' 端为磷酸基团、3' 端为羟基的多聚核苷酸，平均消化产物最小为多聚四核苷酸。
产品特性
1. Mg²⁺存在条件下，DNase I 可随机识别和切断 DNA 任一条链上的任意位点；而在 Mn²⁺存在条件下，DNase I 识别 DNA 的两条链并在几乎相同的位点进行切割；
2. DNase I 可水解多种形式 DNA，如单链 DNA、双链 DNA，甚至染色质（其切割速率受组蛋白影响）。
稳定性
最佳的工作范围是 pH 7-8，DNase 的活性依赖于 Ca²⁺，并可被二价金属离子如 Mn²⁺，Zn²⁺等激活。5mM Ca²⁺可保护酶使其不被水解，而 0.1mM Ca²⁺可使酶失活速率降至原来的 1/2。
应用
1. 制备不含 DNA 的 RNA 样本；
2. RT-PCR 反应前 RNA 样本内基因组 DNA 等可能存在 DNA 污染的去除；
3. T7，T3，SP6 等 RNA 聚合酶催化的体外 RNA 转录后 DNA 模板的去除；
4. 研究 DNA - 蛋白质相互作用；
5. 缺口平移法标记探针用；
6. 制备随机 DNA 片段文库；
7. TUNEL 细胞凋亡检测中用于剪切 DNA 制备阳性对照。
注意事项
为了您的安全和健康，请穿实验服并戴一次性手套操作。
DNase I is a type of endonuclease that can be found in most cells and tissues, preferring to cleave phosphate diester bonds adjacent to pyrimidines, producing polynucleotides with a 5' end phosphate group and a 3' end hydroxyl group. The smallest average digested product is polytetranucleotide.
PROPERTIES
1. Under the presence of Mg²⁺, DNase I can randomly recognize and cleave any site on any strand of DNA; In the presence of Mn²⁺, DNase recognizes two strands of DNA and cleaves them at almost identical sites;
2. DNase I can hydrolyze various forms of DNA, such as single stranded DNA, double stranded DNA, and even chromatin (its cleavage rate is influenced by histones).
STABILITY
The optimal working range is pH 7-8, and the activity of DNase depends on Ca²⁺ and can be activated by divalent metal ions such as Mn²⁺, Zn²⁺, etc. 5mM Ca²⁺ can protect the enzyme from hydrolysis, while 0.1mM Ca²⁺ can reduce the enzyme inactivation rate to half.
APPLICATION
1. Prepare RNA samples without DNA;
2. Removal of possible DNA contamination in RNA samples prior to RT-PCR reaction, including genomic DNA;
3. Removal of DNA templates after in vitro RNA transcription catalyzed by RNA polymerase enzymes such as T7, T3, SP6, etc;
4. Study DNA protein interactions;
5. Notch translation method for labeling probes;
6. Preparation of a random DNA fragment library;
7. Used in TUNEL cell apoptosis detection to prepare positive controls by splicing DNA.
ATTENTION
For your safety and health, please wear a lab coat and disposable gloves.
K2429480
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