Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.
Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

规格

产品名称

DNA酶I

别名

脱氧核糖核酸酶 I

规格或纯度

EnzymoPure™, ≥2,000 Kunitz units/mg dry weight；来源于牛胰腺

生化机理

DNase I 是一种内切酶，可作用于嘧啶附近的磷酸二酯键，生成末端带有 5′-磷酸的多核苷酸。在 Mg2+ 存在的情况下，DNAse I 可独立裂解 DNA 的每一条链，裂解位点是随机的。在 Mn2+ 存在的情况下，两条 DNA 链会在大致相同的部位被裂解。Mn2+、Ca2+、Co2+ 和 Zn2+ 等二价阳离子是该酶的活化剂。浓度为 5 mM 的 Ca2+ 能使酶稳定，防止蛋白水解。最佳 pH 值为 7 至 8。牛胰腺中的 DNAse I 由 A、B、C 和 D 四种色谱法可区分的成分组成，它们的摩尔比分别为 4:1:1。其中只有少量的 D。已知 2-巯基乙醇、螯合剂、十二烷基硫酸钠（SDS）6 和肌动蛋白7 可抑制酶的活性。

CAS编号和信息

9003-98-9

酶学委员会编号

3.1.21.1

分子类型

酶

储存与运输

浓度

≥2,000 Kunitz units/mg dry weight；来源于牛胰腺

储存条件

2-8°C储存

运输条件

低温运输

单位定义

1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Aladdin. A

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