胰蛋白酶 来源于人类胰腺

CAS: 9002-07-7 货号: np001008 EC号: 607-831-3
有货
级别和纯度: 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 ≥95%(SDS-PAGE) Pre-lyophilization Protein Concentration
Accession #
P35030,P07478,P07477
生物活性
≥2.5 U/mg protein
★
规格
库存
价格
数量
25μg
np001008-25μg
现货 Stock Image
¥2,699.90
100μg
np001008-100μg
现货 Stock Image
¥5,399.90
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为什么选择此级别

天然,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。

概述

水解涉及 L - 精氨酸或 L - 赖氨酸羧基的肽键、酰胺键与酯键。研究发现,囊性纤维化(cystic fibrosis)患者体内该酶及其酶原(胰蛋白酶原,trypsinogen)的血清水平会升高。胰蛋白酶原存在三种亚型,分别是阳离子型(胰蛋白酶原 1,trypsinogen 1)、阴离子型(胰蛋白酶原 2,分子量 22,500)和中胰蛋白酶原(胰蛋白酶原 3,分子量 25,000)。 本品为阳离子型。
本产品由特定组织制备而成,该组织经美国食品药品监督管理局(FDA)要求的检测证实,乙型肝炎表面抗原(HBsAg)、丙型肝炎抗体(anti-HCV)、乙型肝炎核心抗体(anti-HBc)检测结果均为阴性,1 型和 2 型人类免疫缺陷病毒抗体(anti-HIV 1 & 2)检测结果也为阴性。
阿拉丁(Aladdin)产品均为实验室试剂,不得用于人体给药,也不得用于任何药物相关用途。仅限科研或进一步生产使用。

Hydrolyzes peptides, amides, and ester bonds involving the carboxyl groups of L-arginine or L-lysine. Increased serum values of this enzyme and/or its zymogen (trypsinogen) have been found in patients with cystic fibrosis. Trypsinogen has three isoforms: cationic (trypsinogen 1), anionic (trypsinogen 2, MW 22,500), and mesotrypsinogen (trypsinogen 3, MW, 25,000). This product is the cationic form.
Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Aladdin products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

规格

产品名称
胰蛋白酶 来源于人类胰腺
英文别名
Serine Protease 1 | PRSS1 | PRSS2 | PRSS3 | TRP1 | TRY1 | TRY4 | TRYP1 | TRY2 | TRY8 | TRYP2 | T9 | MTG | TRY3 | PRSS4
规格或纯度
生物活性, 天然, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration
生化机理
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. Post-translational: Sulfated on tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.Phosphorylation sites are present in the extracellular medium.
生物活性
≥2.5 U/mg protein
种属
人(Human)
来源
天然
预测分子量
21-23 kDa
CAS编号和信息
9002-07-7
酶学委员会编号
3.4.21.4
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 2 mM HCl.
浓度
Pre-lyophilization Protein Concentration
复溶
After centrifugation, we suggest addition of 2 mM HCl to the original volume (See COA), followed by gentle mixingto ensure adequate homogenization.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(>1年);复溶后建议分装,避免反复冻融。
单位定义
One unit is defined as the amount of enzyme that hydrolyzes one umole of N-benzoyl-DL-arginine-pNA per minute at 25oC in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ25F1129425 分析证书 np001008
ZJ25F1129424 分析证书 np001008
技术文档和文章
重组胰蛋白酶
Recombinant Trypsin
纯净提取,智慧溶剂:天然提取试剂实践手册
Clean Extracts, Smart Solvents: The Natural Extraction Reagent Playbook
BRET(生物发光共振能量转移)方法
BRET (Bioluminescence Resonance Energy Transfer) Method
酶保护剂及延长酶活性的常用方法
Enzyme Protectants and Common Methods to Prolong Enzymatic Activity
红细胞裂解液技术说明与操作指南
Red Blood Cell Lysis Buffer — Technical Description & Operating Guide
胰蛋白酶与重组胰蛋白酶药典标准比较
Comparison of Pharmacopoeial Standards for Trypsin and Recombinant Trypsin
肽图检查法鉴定蛋白质结构
Peptide Mapping for Protein Structure Identification
CCK-8细胞活性检测实验方案
CCK-8 Cell Viability Assay Protocol
LPS诱导细胞炎症模型建立实验步骤
LPS-Induced Cellular Inflammation Model: Experimental Procedure
EdU染色实验的基本原理和实验流程
EdU Staining: Basic Principle and Experimental Workflow
HepG2细胞脂肪变性模型建立实验流程
HepG2 Cell Steatosis Model Establishment Protocol
骨髓间充质干细胞分离培养及诱导分化为破骨细胞实验操作流程
Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure
关于细胞培养的常见问答
Frequently Asked Questions about Cell Culture
多种组织单细胞解离酶的应用与优化
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
常见组织解离酶的原理与选择策略
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
抑肽酶技术综述:结构特征、作用机制与应用
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
姬姆萨(Giemsa)染色实验Protocol:血液涂片与骨髓涂片
Giemsa Staining Protocol: Peripheral Blood and Bone Marrow Smears
赖氨酰内切酶Lys-C:赖氨酸位点特异性消化酶的机理、应用与方法学控制要点
Endoproteinase Lys-C: Mechanism, Applications, and Method-Control Considerations for Lysine-Specific Digestion
科研常用蛋白酶的基础介绍与典型应用
Commonly Used Proteases in Research: Fundamentals and Representative Applications
植物组学技术全景:基因组—转录组—蛋白质组—代谢组的流程框架与多组学整合应用
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
乳清蛋白:组成谱、制备路径与产品类型要点
Whey Protein: Composition Profile, Manufacturing Routes, and Key Product-Type Essentials
弗林蛋白酶(Furin)的结构特征、底物识别与科研应用综述
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
羧肽酶家族在蛋白末端加工与实验分析中的作用
Roles of the Carboxypeptidase Family in Protein C-Terminal Processing and Experimental Analysis
糖蛋白结构表征与质量属性分析的方法学研究
Methodological Studies on Glycoprotein Structural Characterization and Quality Attribute Analysis
组织单细胞制备的样本处理与质控策略
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
聚电解质多层膜的研究价值:界面组成、传质与功能的逐层设计
Research Value of Polyelectrolyte Multilayers: Layer-by-Layer Design of Interfacial Composition, Mass Transport, and Function
IHC实验中低pH、高pH及酶法抗原修复体系的应用比较
Comparison of Low-pH, High-pH, and Enzymatic Antigen Retrieval Systems in IHC
常用哺乳动物细胞抗性筛选体系比较:嘌呤霉素、G418、潮霉素B与杀稻瘟菌素
Comparison of Common Mammalian Cell Antibiotic Selection Systems: Puromycin, G418, Hygromycin B, and Blasticidin
内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
生物模拟肽的皮肤作用逻辑:序列与构象、皮肤递送、稳定性及靶点匹配
The Logic of Biomimetic Peptide Action in Skin: Sequence and Conformation, Skin Delivery, Stability, and Target Matching
丝氨酸内切蛋白酶的催化机制、分类特征与实验应用
Catalytic Mechanisms, Classification Characteristics, and Experimental Applications of Serine Endopeptidases
此产品的引用文献
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