K2513240计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
有货
为什么选择此级别
EnzymoPure™,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 34 篇同行评审文献引用。
概述
Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.
规格
产品名称
胶原蛋白酶 来源于溶组织梭菌(类型4)
别名
梭菌肽酶 A | 胶原酶
英文别名
Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene | microbial c
规格或纯度
天然, EnzymoPure™, ≥160 units/mg dry weight
生物活性
≥160 units/mg dry weight
种属
溶组织梭菌(Clostridium histolyticum)
Accession #
来源
天然
CAS编号和信息
9001-12-1
酶学委员会编号
EC 3.4.24.3
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥160 units/mg dry weight
储存条件
2-8°C储存
运输条件
低温运输
单位定义
One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5.
技术文档
高级数据
关联靶点（人）
HT-29
(80576 活性数据)
MCF7
(126967 活性数据)
SK-MEL-5
(47095 活性数据)
A549
(127892 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
多种组织单细胞解离酶的应用与优化
目录： 技术文章
Photo-Crosslinking and Patterning of PEG-Based Hydrogels (2D/3D): Principles, Method Comparisons, and Product Selection Guidelines
目录： 技术文章
Cholesterol Esterase: Mechanistic Insights and Application Frameworks Driven by Interfacial Hydrolysis
目录： 技术文章
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
目录： 技术文章
Designing Hydrogel Scaffolds as Programmable Cell Microenvironments: From Click-Chemistry Functionalization to 3D Cell Culture Experimental Design
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
Culture Systems, Molecular Markers, and Research Applications of Major Human Organ Organoids
目录： 技术文章
Culture Systems, Phenotypic Characterization, and Translational Research Applications of Tumor Organoids
目录： 技术文章
How Palmitoyl Pentapeptide-4 Acts on the Skin: From the KTTKS Sequence and Skin Delivery to Collagen Matrix Responses
目录： 技术文章
How Does Collagen Tripeptide Act on the Skin: Stratum Corneum Delivery, Matrix Protection, and Regulation of Collagen Homeostasis
目录： 技术文章
What Determines Collagen Function: From Triple-Helix Formation to Hierarchical Assembly, Crosslink Maturation, and Dynamic Remodeling
目录： 技术文章
What Is “Plant Collagen”? Distinguishing Ingredient Types, Structural Differences, Production Methods, and Skincare Effects
目录： 技术文章
此产品的引用文献
引用文献
| 1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, [PMID:37421781] [10.1016/j.biopha.2023.115096] |
| 2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:37178890] [10.1016/j.ijbiomac.2023.124820] |
| 3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, [10.1016/j.colsurfa.2023.131410] |
| 4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2022) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, [PMID:36521639] [10.1016/j.ijpharm.2022.122489] |
| 5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2022) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, [PMID:36471892] [10.1016/j.mtbio.2022.100506] |
| 6. Congying Wei, Weili Shi, Chuangqi Zhao, Shuai Yang, Jiajia Zheng, JinPan Zhong, Tianyi Zhao, Simin Kong, Xi Gong, Mingjie Liu. (2022) Superwetting Injectable Hydrogel with Ultrastrong and Fast Tissue Adhesion for Minimally Invasive Hemostasis. Advanced Healthcare Materials, 12 (3): (2201799). [PMID:36333905] [10.1002/adhm.202201799] |
| 7. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [10.1088/1361-665X/ac884a] |
| 8. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, [PMID:32663418] [10.1080/09205063.2020.1796229] |
| 9. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2019) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, [PMID:32228945] [10.1016/j.msec.2019.110538] |
| 10. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068] |
| 11. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, [PMID:31029343] [10.1016/j.msec.2019.03.093] |
| 12. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, [PMID:30184770] [10.1016/j.msec.2018.06.072] |
| 13. Liuxin Yang, Fengya Jing, Dandan Wei, Xiaocong Zhao, Yinghua Tao, Tao Liu, Tianzhu Zhang. (2025) Assembled granular hydrogels loaded with growth factors for enhanced mesenchymal stem cell therapy in abdominal wall defect repair. JOURNAL OF CONTROLLED RELEASE, [PMID:40090523] [10.1016/j.jconrel.2025.113630] |
| 14. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao. (2024) Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo. INDUSTRIAL CROPS AND PRODUCTS, [10.1016/j.indcrop.2024.118716] |
| 15. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, [PMID:38949962] [10.1021/acsnano.4c02921] |
| 16. Chen Zhengfang, He Mudan, Wang Houpeng, Li Xuehui, Qin Ruirui, Ye Ding, Zhai Xue, Zhu Junwen, Zhang Quanqing, Hu Peng, Shui Guanghou, Sun Yonghua. (2024) Intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally deposited yolk lipids. Nature Communications, 15 (1): (1-15). [PMID:39528516] [10.1038/s41467-024-54258-2] |
| 17. Liuxin Yang, Dandan Wei, Yinghua Tao, Fengya Jing, Tao Liu, Guanhua Jiao, Tianzhu Zhang. (2024) Spatiotemporal delivery of microenvironment responsive hydrogel incorporated with stem cells-loaded porous microspheres for abdominal wall repair. CHEMICAL ENGINEERING JOURNAL, [10.1016/j.cej.2024.154435] |
| 18. Liuxin Yang, Guanhua Jiao, Dandan Wei, Tianzhu Zhang. (2025) Synthesis and characterization of photocrosslinked gelatin-silk fibroin composite microspheres for mesenchymal stem cell expansion and osteogenic differentiation. Materials Today Communications, [10.1016/j.mtcomm.2025.111912] |
| 19. Jianping Zhao, Di Zhang, Qiumei Lan, Gang Zhong, Yisi Liu, Nathaniel Holwell, Xiang Wang, Jinzhi Meng, Jun Yao, Brian G. Amsden, Yin Yu, Fei Chen. (2024) Tendon Decellularized Matrix Modified Fibrous Scaffolds with Porous and Crimped Microstructure for Tendon Regeneration. ACS Applied Bio Materials, [PMID:39005189] [10.1021/acsabm.4c00565] |
| 20. Yun Liu, Haibo Zhao, Yishan Fang, Zhengzong Wu, Bin Yu, Bo Cui. (2024) Hydrogels of dialdehyde starch and gelatin cross-linked with potential application as tissue adhesives. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39706428] [10.1016/j.ijbiomac.2024.138956] |
| 21. Shenghao Yang, Zhongrui Wang, Yuwen Meng, Xu Wang. (2024) Preparation and properties of biphasic hydrogels with tunable microstructures. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, [10.1016/j.colsurfa.2024.135464] |
| 22. Subramani Kandhasamy, Baofang Wu, Jiayin Wang, Xiaojing Zhang, Gao Hongzhi, Da-Peng Yang, Yiming Zeng. (2024) Tracheal regeneration and mesenchymal stem cell augmenting potential of natural polyphenol-loaded gelatinmethacryloyl bioadhesive. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:38772466] [10.1016/j.ijbiomac.2024.132506] |
| 23. Hua Chai, Renpeng Xia, Nan Ouyang, Jingyu Yao, Jiukun Hu, Minxuan Sun, Peng Miao. (2025) Membrane Encapsulated Fe3O4@MnO@cGAMP Nanocatalyst for Cancer Therapy: cGAS-STING Immune Pathway Activation. Advanced Healthcare Materials, [PMID:40231491] [10.1002/adhm.202501587] |
| 24. Yajie Cheng, Yadong Chen, Chang Shu, Chenying Zhou, Zhenzhen Zhang, Yan Tu, Qiaojie Luo, Xiaodong Li. (2025) Facile Ag+ assisted bonding strategy built a defect-low hybrid layer with intrinsic antibacterial and enzymolysis-inhibitory property. Journal of Materials Chemistry B, [PMID:40469027] [10.1039/D5TB00584A] |
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| 30. He Haisheng, Zhu Wenwei, Miao Han, Wang Shangfeng, Du Zunguo, Zhang Hongxin, Ming Jiang, Shi Ben, Wang Hao, Qi Jianping, Fan Yong, Wu Wei, Zhao Dongyuan, Qin Lun-Xiu, Zhang Fan. (2026) Label-free tissue NIR-II autofluorescence imaging for visualization of human liver malignancy. Nature Biomedical Engineering, [PMID:41559191] [10.1038/s41551-025-01593-4] |
| 31. Li Cai, Jian Gao, Kai Zhang, Bing Xiao, SiJia Xu, Wei Zhao, Juan Li, Yanli Zhou, WenYing Zhu, ShuYuan Liu, TingTing Pei, JunHua Li, Yang Chen, ShiXian Chen, Ji Li, Juan Li. (2026) Hydrogel microspheres loaded with sinomenine and Drynaria rhizome enhance the treatment of rheumatoid arthritis via immune regulation and promoting bone repair. JOURNAL OF NANOBIOTECHNOLOGY, [PMID:41582151] [10.1186/s12951-026-04063-4] |
| 32. Ye Ding, Hao Yongkang, Zhu Junwen, Wang Chaofan, Li Linglu, Li Yongming, Tu Yi-Xuan, Ren Zhiqin, Wang Houpeng, Chen Zhengfang, He Mudan, Wang Xiaosi, Sun Yonghua. (2026) Ultra-fast generation of all-female grass carp via transplantation of female germline stem cells into zebrafish. Science China-Life Sciences, [PMID:41866455] [10.1007/s11427-026-3272-2] |
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溶液计算器
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