计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,His标签,分子生物学级,无动物源,无菌过滤,无载体,生物活性 级 提供 ≥90% 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。
产品介绍:
胰蛋白酶是一种丝氨酸蛋白酶,可特异切割赖氨酸及精氨酸C末端肽键。本产品是由微生物基因重组表达生产,氨基酸序列与猪胰腺来源的胰蛋白酶一致,重组猪胰蛋白酶具有与动物源性猪胰蛋白酶相同的酶学性质,可替代猪胰腺来源胰蛋白酶应用于各种生物技术过程中,如:重组胰岛素生产、细胞培养、细胞发酵、蛋白质的酶解以及各种组织的细胞分离等。胰蛋白酶活性受丝氨酸蛋白酶抑制剂如PMSF等的抑制,金属离子螯合剂如EDTA等也能抑制酶活。
产品特性:
酶活:>10000 U/mg 冻干粉
单位定义:25℃,pH7.6,反应体系3.2mL(1cm光路),每分钟酶解BAEE使253nm下的吸收值增加0.001定义为一个胰蛋白酶酶活单位(U)
溶 解 性: >20 mg/mL
纯 度:≥70% β-胰蛋白酶,≤20% α-胰蛋白酶
蛋白含量: ≥90%
DNA :<1 ppm
支 原 体:未检出
内 毒 素:≤10 EU/mg
微生物限度:≤5 cfu/g
最适pH范围:7-11
最适温度范围:推荐温度范围是25-37℃,温度为37℃活性最高
Product Introduction:
Trypsin is a serine protease, which can specifically cleave the C-terminal peptide bond of lysine and arginine. This product is produced by recombinant expression of microbial genes, and its amino acid sequence is consistent with that of porcine pancreatic trypsin. The recombinant porcine trypsin has the same enzymatic properties as that of porcine pancreatic trypsin from animals, and can be used in various biotechnology processes instead of porcine pancreatic trypsin, such as recombinant insulin production, cell culture, cell fermentation, protein enzymolysis and cell separation of various tissues. Trypsin activity is inhibited by serine protease inhibitors such as PMSF, and metal ion chelating agents such as EDTA can also inhibit enzyme activity.
Product Features:
Enzyme activity: >10000 U/mg freeze-dried powder
Unit definition: 25℃, pH7.6, reaction system 3.2mL(1cm light path), enzymatic hydrolysis of BAEE per minute increases the absorption value at 253nm by 0.001, which is defined as a trypsin activity unit (U).
Solubility: >20 mg/mL
Purity: ≥70% β -trypsin and ≤20% α -trypsin
Protein content: ≥90%
DNA :<1 ppm
Mycoplasma: Not detected.
Endotoxin: ≤10 EU/mg
Microbial limit: ≤5 cfu/g
Optimum pH range: 7-11.
Optimum temperature range: the recommended temperature range is 25-37℃, and the activity is the highest at 37℃.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 | |
| 分析证书 | rp212900 |
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查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 Sterile-filtered 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 EnzymoPure™ 级别指南 → 查看 His Tag 级别指南 →