计算溶液所需的质量、体积或浓度。
聚(乙二醇)(PEG)是非离子型亲水聚合物,并且可以获得不同的分子量形式。它有助于蛋白质和核酸的纯化和晶体生长。PEG可与葡聚糖一起产生含水聚合物两相体系,该体系是生物材料纯化所必需的。PEG还能与细胞膜相互作用,从而使得细胞融合。
应用
聚(乙二醇)已用于Galegae orientalis幼苗的胁迫(脱水)处理。它已被用于制备水性两相系统以研究跨界面的蛋白质扩散。
其他说明
蛋白质的分级沉淀;原生质体融合;病毒纯化;在含有PEG和抗体的琼脂糖凝胶上进行蛋白电印迹
Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.
Application
Poly(ethylene glycol) has been used for stress (dehydration) treatment in seedlings of Galegae orientalis. It has been used for the preparation of aqueous two-phase system to study protein diffusion across the interface.
Other Notes
Fractional precipitation of proteins; Protoplast fusion; In virus purification; Electroblotting of proteins on agarose gel containing PEG and antibodies
| pH | 5.5-7.0 (25 °C, 50 mg/mL in H2O) |
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