计算溶液所需的质量、体积或浓度。
EnzymoPure™,PBS Only,无动物源,无载体,质谱级 (MS),重组 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。
蛋白组学分析中可靠且可重复的结果高度依赖于稳定的样品制备,样品的制备过程繁琐耗时,通常包括16-18小时的过夜孵育酶解过程,在酶解过程中平衡速度与酶切完整性是一个持续的挑战。快速胰蛋白酶(质谱级)属于质谱级别的基因工程酶,能够实现在3小时内完成蛋白组学研究中的酶解过程,其能够特异性地切割赖氨酸(K)及精氨酸(R)酸C末端肽键,适用于多种类型样本,包括但不限于动物组织来源样本、细胞样本及IP样本。
产品用途
可用于肽图谱分析、蛋白序列分析以及蛋白组学研究中的蛋白酶解过程。
产品特点
高效酶解:酶解时间缩短至3 h以内,节约了样品前处理的时间。
高纯度:无其他污染蛋白酶,纯度≥95%。
高稳定性:每批产品经严格的质量控制,保证产品批次间稳定性。
无动物源性:本产品为重组表达,生产过程中不使用任何动物源的材料,无外源性的病毒污染。
产品数据

图1. 结果表明Rapid-Trypsin在3 h完成酶切
组分表
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操作流程
①推荐使用50mM的醋酸溶液溶解Rapid-Trypsin (MS)冻干粉。比如:取1管20μg的Rapid-Trypsin (MS)冻干粉,加入40μL 50mM的醋酸溶液溶解,可进行Votex震荡溶解,配置成0.5μg/uL的Rapid-Trypsin (MS)溶液,每管5μL,分装成8管保存。
②Rapid-Trypsin (MS)推荐的使用比例为Rapid-Trypsin (MS) :目的蛋白=1:20,该比例为质量比,比如1μg的Rapid-Trypsin (MS)可以切20μg的总蛋白。(推荐酶切时间为3-4h,对于较难消化的蛋白或复杂样品,酶切时间可延长至6h。)
注意事项和免责声明
本品仅限于专业人员的科学研究使用,不得用于临床诊断或治疗,不得用于食品或药品。
In proteomic analysis, reliable and reproducible results are highly dependent on stable sample preparation. The sample preparation process is cumbersome and time-consuming, usually involving an overnight incubation and enzymatic hydrolysis process of 16-18 hours. Balancing speed and enzymatic cleavage completeness during the enzymatic hydrolysis process is an ongoing challenge. Fast Trypsin (Mass Spectrometry Grade) is a mass spectrometry-grade genetic engineering enzyme that can complete the enzymatic hydrolysis process in proteomic research within 3 hours. It can specifically cleave the C-terminal peptide bonds of lysine (K) and arginine (R), and is suitable for various types of samples, including but not limited to samples derived from animal tissues, cell samples, and IP samples.
Product Applications
It can be used in peptide mapping analysis, protein sequence analysis, and the proteolytic process in proteomic research.
Product Features
Efficient enzymatic hydrolysis: The enzymatic hydrolysis time is shortened to less than 3 hours, saving the time for sample pretreatment.
High purity: Free of other contaminating proteases, with a purity of ≥95%.
High stability: Each batch of products undergoes strict quality control to ensure stability between batches.
Animal origin-free: This product is recombinantly expressed, and no animal-derived materials are used in the production process, thus avoiding exogenous virus contamination.
Product Data

Fig. 1. The results showed that Rapid-Trypsin (MS) completed the enzyme digestion in 3 h.
Component List
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Operating Procedures
① It is recommended to dissolve the Rapid-Trypsin (MS) lyophilized powder using 50mM acetic acid solution. For example: Take 1 tube of 20μg Rapid-Trypsin (MS) lyophilized powder, add 40μL of 50mM acetic acid solution, and dissolve by vortexing to prepare a 0.5μg/μL Rapid-Trypsin (MS) solution. Aliquot into 8 tubes, 5μL per tube, for storage.
② The recommended usage ratio of Rapid-Trypsin (MS) is Rapid-Trypsin (MS) : target protein = 1:20 (mass ratio). For instance, 1μg of Rapid-Trypsin (MS) can digest 20μg of total protein. (The recommended digestion time is 3-4 hours; for hard-to-digest proteins or complex samples, the digestion time can be extended to 6 hours.)
Precautions and Disclaimer
This product is limited to scientific research use by professionals. It must not be used for clinical diagnosis or treatment, nor for food or pharmaceutical purposes.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | R1456327 | |
| 分析证书 | R1456327 | |
| 分析证书 | R1456327 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Recombinant 级别指南 → 查看 Suitable for mass spectrometry (MS) 级别指南 → 查看 EnzymoPure™ 级别指南 → 查看 PBS Only 级别指南 →