计算溶液所需的质量、体积或浓度。
有货
Accession #
P00760
为什么选择此级别
EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。
概述
胰蛋白酶是一种丝氨酸蛋白水解酶,属白色或微黄色冻干粉制品。它能特异性地水解碱性氨基酸精氨酸及赖氨酸羧基所组成的肽键。酶本身很容易自溶，其作用最佳的PH值为8.0～9.0。胰蛋白酶活性能被胰腺中的天然抑制剂所抑制。钙离子能延迟胰蛋白酶的自溶，并能促进胰蛋白酶原的活性。or trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns?. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin is a single chain polypeptide of 223 amino acid residues and is a member of the serine protease family. The active site amino acid residues of trypsin include HIs46 and Ser183. Trypsin is produced by removing the N-terminal hexapeptide from trypsinogen which is cleaved at the peptide bonds at Lys6 - lle7. This cleavage yields a single chain native form of trypsin called β-Trypsin. Ensuing autolysis of β-Trypsin results in α-Trypsin having two peptide chains bound by disulphide bonds. This enzyme predominantly cleaves peptide chains at the carboxyl side of Arginine and Lysine, with an exception when either one is followed by a Proline.
or trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns?. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
规格
产品名称
胰蛋白酶（牛胰脏）
规格或纯度
EnzymoPure™, potency ≥2500 units/mg
生化机理
胰蛋白酶可在赖氨酸和精氨酸残基的 C 端侧裂解肽。如果酸性残基位于裂解位点的两侧，水解速度就会减慢；如果脯氨酸残基位于裂解位点的羧基侧，水解就会停止。胰蛋白酶活性的最佳 pH 值为 7-9。胰蛋白酶还能裂解氨基酸合成衍生物的酯和酰胺连接。在胰蛋白酶溶液中加入乙二胺四乙酸（EDTA）作为螯合剂，可中和钙镁离子，因为钙镁离子会遮蔽胰蛋白酶作用的肽键。去除这些离子可提高酶的活性。
CAS编号和信息
9002-07-7
分子类型
酶
储存与运输
物理形态
冻干(Lyophilized)
浓度
potency ≥2500 units/mg
储存条件
避光,-20°C储存
运输条件
超低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 E2308147 |
分析证书 | T105531 |
| I2601049 |
分析证书 | T105531 |
| H2612070 |
分析证书 | T105531 |
| F2626025 |
分析证书 | T105531 |
| H2413502 |
分析证书 | T105531 |
| E2428369 |
分析证书 | T105531 |
| E2428370 |
分析证书 | T105531 |
| G2220656 |
分析证书 | T105531 |
| G2220659 |
分析证书 | T105531 |
| B2422207 |
分析证书 | T105531 |
| C2507152 |
分析证书 | T105531 |
| H2413504 |
分析证书 | T105531 |
| H2413505 |
分析证书 | T105531 |
| F2523046 |
分析证书 | T105531 |
| E2428368 |
分析证书 | T105531 |
| B2422208 |
分析证书 | T105531 |
| A2431109 |
分析证书 | T105531 |
| I2110298 |
分析证书 | T105531 |
| I2110299 |
分析证书 | T105531 |
| I2110297 |
分析证书 | T105531 |
| D2101194 |
分析证书 | T105531 |
| G2220660 |
分析证书 | T105531 |
技术文档和文章
酶保护剂及延长酶活性的常用方法
目录： 技术文章
肽图检查法鉴定蛋白质结构
目录： 技术文章
Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure
目录： 实验方案（Protocols）
多种组织单细胞解离酶的应用与优化
目录： 技术文章
常见组织解离酶的原理与选择策略
目录： 技术文章
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
目录： 技术文章
Endoproteinase Lys-C: Mechanism, Applications, and Method-Control Considerations for Lysine-Specific Digestion
目录： 技术文章
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
目录： 技术文章
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
目录： 技术文章
Roles of the Carboxypeptidase Family in Protein C-Terminal Processing and Experimental Analysis
目录： 技术文章
Methodological Studies on Glycoprotein Structural Characterization and Quality Attribute Analysis
目录： 技术文章
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
目录： 技术文章
Research Value of Polyelectrolyte Multilayers: Layer-by-Layer Design of Interfacial Composition, Mass Transport, and Function
目录： 技术文章
Comparison of Common Mammalian Cell Antibiotic Selection Systems: Puromycin, G418, Hygromycin B, and Blasticidin
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
此产品的引用文献
溶液计算器
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