胰蛋白酶 来源于牛胰腺(纯化,测序级II)

CAS: 9002-07-7 货号: T128770 EC号: 607-831-3
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级别和纯度: 质谱级 (MS) ? 适用于质谱(MS)—— 非挥发性和离子残留低,与质谱兼容。用于在质谱流程中尽量降低背景和离子抑制。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 ≥150 units/mg protein (at least 8,625 BAEE/2875 USP/NF units/mg protein)
Accession #
P00760
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规格
库存
价格
数量
25μg
T128770-25μg
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¥881.90
100μg
T128770-100μg
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¥2,471.90
1mg
T128770-1mg
期货 Stock Image
¥11,249.90
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为什么选择此级别

EnzymoPure™,质谱级 (MS) 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。

概述


Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.
Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins

规格

产品名称
胰蛋白酶 来源于牛胰腺(纯化,测序级II)
英文别名
Peptidyl peptide hydrolase
规格或纯度
EnzymoPure™, 质谱级 (MS), ≥150 units/mg protein (at least 8,625 BAEE/2875 USP/NF units/mg protein)
生化机理
胰蛋白酶主要在氨基酸赖氨酸或精氨酸的羧基侧裂解肽链、酰胺和酯。胰蛋白酶以酶原胰蛋白酶原的形式存在于胰腺中。
CAS编号和信息
9002-07-7
酶学委员会编号
I.U.B.: 3.4.21.4
分子类型
酶
储存与运输
物理形态
固体
浓度
≥150 units/mg protein (at least 8,625 BAEE/2875 USP/NF units/mg protein)
储存条件
-20°C储存,充氩
运输条件
超低温运输
单位定义
Unit Definitions: TAME Unit: One Unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.

技术文档

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
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找到7个结果

批号(Lot Number) 证书类型 货号
H2507740 分析证书 T128770
B2517118 分析证书 T128770
A2514405 分析证书 T128770
L2403463 分析证书 T128770
K1822175 分析证书 T128770
D2230045 分析证书 T128770
D2230063 分析证书 T128770
技术文档和文章
先进的酶分析技术
Advanced enzyme analysis technology
用胰蛋白酶解离细胞
Dissociation of cells with trypsin
蛋白样品制备流程
Protein sample preparation process
流式实验样品制备实验指南
Experimental Guide of Sample Preparation for Flow Cytometry
CUT&RUN-seq实验方案
利用血细胞计数器计数细胞法
Methods for Counting Cells Using a Hemocytometer
哺乳动物细胞冷冻保存方案
Mammalian Cell Cryopreservation Protocol
MTT检测方案
MTT Assay Protocol
重组胰蛋白酶
Recombinant Trypsin
简介:“适用于质谱分析” (又名:质谱级)
Introduction: “Suitable for Mass Spectrometry”
质谱级 (MS)
MS Grade
BRET(生物发光共振能量转移)方法
BRET (Bioluminescence Resonance Energy Transfer) Method
酶保护剂及延长酶活性的常用方法
Enzyme Protectants and Common Methods to Prolong Enzymatic Activity
红细胞裂解液技术说明与操作指南
Red Blood Cell Lysis Buffer — Technical Description & Operating Guide
胰蛋白酶与重组胰蛋白酶药典标准比较
Comparison of Pharmacopoeial Standards for Trypsin and Recombinant Trypsin
肽图检查法鉴定蛋白质结构
Peptide Mapping for Protein Structure Identification
质谱级酶:精准蛋白质组学分析的“分子剪刀”
MS-Grade Enzymes:“Molecular Scissors” for Precision Proteomics Analysis
CCK-8细胞活性检测实验方案
CCK-8 Cell Viability Assay Protocol
LPS诱导细胞炎症模型建立实验步骤
LPS-Induced Cellular Inflammation Model: Experimental Procedure
EdU染色实验的基本原理和实验流程
EdU Staining: Basic Principle and Experimental Workflow
HepG2细胞脂肪变性模型建立实验流程
HepG2 Cell Steatosis Model Establishment Protocol
骨髓间充质干细胞分离培养及诱导分化为破骨细胞实验操作流程
Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure
关于细胞培养的常见问答
Frequently Asked Questions about Cell Culture
多种组织单细胞解离酶的应用与优化
Applications and Optimization of Multi-Tissue Single-Cell Dissociation Enzymes
常见组织解离酶的原理与选择策略
Principles and Selection Strategies for Common Tissue Dissociation Enzymes
抑肽酶技术综述:结构特征、作用机制与应用
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
姬姆萨(Giemsa)染色实验Protocol:血液涂片与骨髓涂片
Giemsa Staining Protocol: Peripheral Blood and Bone Marrow Smears
赖氨酰内切酶Lys-C:赖氨酸位点特异性消化酶的机理、应用与方法学控制要点
Endoproteinase Lys-C: Mechanism, Applications, and Method-Control Considerations for Lysine-Specific Digestion
科研常用蛋白酶的基础介绍与典型应用
Commonly Used Proteases in Research: Fundamentals and Representative Applications
植物组学技术全景:基因组—转录组—蛋白质组—代谢组的流程框架与多组学整合应用
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
乳清蛋白:组成谱、制备路径与产品类型要点
Whey Protein: Composition Profile, Manufacturing Routes, and Key Product-Type Essentials
弗林蛋白酶(Furin)的结构特征、底物识别与科研应用综述
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
羧肽酶家族在蛋白末端加工与实验分析中的作用
Roles of the Carboxypeptidase Family in Protein C-Terminal Processing and Experimental Analysis
糖蛋白结构表征与质量属性分析的方法学研究
Methodological Studies on Glycoprotein Structural Characterization and Quality Attribute Analysis
组织单细胞制备的样本处理与质控策略
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
多酶消化策略与蛋白质组学覆盖度优化研究框架
Research Framework for Multi-Enzyme Digestion Strategies and Optimization of Proteomic Coverage
聚电解质多层膜的研究价值:界面组成、传质与功能的逐层设计
Research Value of Polyelectrolyte Multilayers: Layer-by-Layer Design of Interfacial Composition, Mass Transport, and Function
蛋白质羰基检测
Protein Carbonyl Detection
IHC实验中低pH、高pH及酶法抗原修复体系的应用比较
Comparison of Low-pH, High-pH, and Enzymatic Antigen Retrieval Systems in IHC
常用哺乳动物细胞抗性筛选体系比较:嘌呤霉素、G418、潮霉素B与杀稻瘟菌素
Comparison of Common Mammalian Cell Antibiotic Selection Systems: Puromycin, G418, Hygromycin B, and Blasticidin
CUT&RUN-seq protocol
内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
生物模拟肽的皮肤作用逻辑:序列与构象、皮肤递送、稳定性及靶点匹配
The Logic of Biomimetic Peptide Action in Skin: Sequence and Conformation, Skin Delivery, Stability, and Target Matching
丝氨酸内切蛋白酶的催化机制、分类特征与实验应用
Catalytic Mechanisms, Classification Characteristics, and Experimental Applications of Serine Endopeptidases
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