L2123165计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
EnzymoPure™,质谱级 (MS) 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 441 篇同行评审文献引用。
概述
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins
Specificity:
Trypsin cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. If a proline residue is on the carboxyl side of the cleavage site, the cleavage will not occur. If an acidic residue is on either side of the cleavage site, the rate of hydrolysis has been shown to be slower.
Protein Accession Number: P00760
CATH Classification (v. 3.2.0):
Class: Mainly Beta
Architecture: Beta Barrel
Topology: Thrombin, subunit H
Molecular Weight: 23.3 kDa (Theoretical)
Optimal pH: 7.5-8.5 (Koutsopoulos et al. 2007)
Isoelectric Point:
Trypsinogen: pH 9.3 (Walsh and Neurath 1964)
Trypsin: pH 10.5 (Cunningham 1954).
Extinction Coefficient: Trypsinogen: 45,250 cm-1 M-1>
Active Site Residues:
Histidine (H63)
Aspartic acid (D107)
Serine (S200)
Activators: The rate of trypsinogen conversion is enhanced by using lanthanide in place of calcium ions (Gomez et al. 1974).
Inhibitors:
Pancreatic-, soybean-, lima bean-, and egg white- trypsin inhibitors (see section on Trypsin Inhibitors)
DFP
Aprotinin
Ag+
Benzamidine
EDTA
(White and White 1997)
Applications:
Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase
Cell harvesting by “trypsinization”
Mitochondria isolation
in vitro studies of proteins
Removing monolayers of cells from plastic and glass
Various hemagglutination procedures
Sample preparation for flow cytometric DNA analysis
Tryptic mapping
Fingerprinting and sequencing work
Environmental monitoring
Reduction of cell density in tissue culture
Subculturing cells
Cleavage fusion proteins
Generating glycopeptides from purified glycoproteins
(White and White 1997)
规格
产品名称
胰蛋白酶 来源于牛胰腺(修饰,测序级)
英文别名
Peptidyl peptide hydrolase
规格或纯度
EnzymoPure™, 质谱级 (MS), ≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein)
生化机理
胰蛋白酶主要在氨基酸赖氨酸或精氨酸的羧基侧裂解肽链、酰胺和酯。胰蛋白酶以酶原胰蛋白酶原的形式存在于胰腺中。
CAS编号和信息
9002-07-7
酶学委员会编号
3.4.21.4
分子类型
酶
储存与运输
浓度
≥150 units/mg protein(at least 8,625 BAEE/2875 USP/NF units/mg protein)
储存条件
-20°C储存
运输条件
超低温运输
单位定义
TAME Unit: One Unit hydrolyzes 1 µmole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 10 mM calcium.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
酶保护剂及延长酶活性的常用方法
目录： 技术文章
肽图检查法鉴定蛋白质结构
目录： 技术文章
Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure
目录： 实验方案（Protocols）
多种组织单细胞解离酶的应用与优化
目录： 技术文章
常见组织解离酶的原理与选择策略
目录： 技术文章
A Technical Review of Aprotinin: Structural Features, Mechanisms of Action, and Applications
目录： 技术文章
Endoproteinase Lys-C: Mechanism, Applications, and Method-Control Considerations for Lysine-Specific Digestion
目录： 技术文章
Plant Omics Panorama: Workflow Frameworks and Multi-Omics Integration from Genomics to Transcriptomics, Proteomics, and Metabolomics
目录： 技术文章
Furin: A Review of Its Structural Features, Substrate Recognition, and Research Applications
目录： 技术文章
Roles of the Carboxypeptidase Family in Protein C-Terminal Processing and Experimental Analysis
目录： 技术文章
Methodological Studies on Glycoprotein Structural Characterization and Quality Attribute Analysis
目录： 技术文章
Tissue Dissociation for Single-Cell Preparation: Sample Handling and Quality Control Strategies
目录： 技术文章
Research Framework for Multi-Enzyme Digestion Strategies and Optimization of Proteomic Coverage
目录： 技术文章
Research Value of Polyelectrolyte Multilayers: Layer-by-Layer Design of Interfacial Composition, Mass Transport, and Function
目录： 技术文章
Comparison of Common Mammalian Cell Antibiotic Selection Systems: Puromycin, G418, Hygromycin B, and Blasticidin
目录： 技术文章
Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research
目录： 技术文章
此产品的引用文献
溶液计算器
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查看 Suitable for mass spectrometry (MS) 级别指南 → 查看 EnzymoPure™ 级别指南 →相关产品
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