本品为经 TPCK 处理的胰蛋白酶，因此，胰蛋白酶活力更高，糜胰蛋白酶活性被抑制。TPCK-胰蛋白酶能够专一性地切割蛋白分 子中精氨酸和赖氨酸位点，是蛋白质肽段分析重要工具酶之一。
胰蛋白酶分子有两个结构域：一个与酶活性位点和色氨酸残基有关；另一个与8-苯胺基萘-1-磺酸盐结合有关。
用途：
科研试剂，广泛应用于分子生物学，药理学等科研方面,严禁用于人体。胰蛋白酶消化肽，使用约 1:100 至 1:20 的比例胰蛋白酶：肽。该产品的典型用途是从培养表面去除粘附细胞。从细胞基质中移出细胞所需的胰蛋白酶浓度主要取决于细胞类型和培养时间。胰蛋白酶也被用于细胞悬浮培养过程中的细胞悬浮，蛋白质组学研究，用于消化蛋白质和各种凝胶消化。其他应用包括通过基于膜的技术评估结晶，以及用于确定蛋白质折叠速率和产量可能受到动力学陷阱的存在的限制的研究。
| 单位定义 |
1 BTEE 单位 = 320 ATEE 单位 |
| 来源 |
牛胰腺 |
| CAS No. |
'9002-07-7 |
| 储存条件 |
-20℃，避光防潮密闭干燥 |
| 分子量 |
23.8 kDa |
| 溶解性(25°C) |
hydrochloric acid: soluble 1 mM |
| 注意事项 |
溶解性是在室温下测定的，如果温度过低，可能会影响其溶解性。 |
| 其他说明 |
为了您的安全和健康，请穿实验服并戴一次性手套操作。 | |
使用方法推荐：
一：储存液的配制，用于细胞培养相关实验 ：按照表格里溶解性溶解，如用于细胞实验,请配制成液体之后 用 0.22um 过滤后再加入细胞。
二：储存液的保存：建议现配现用，液体不是很稳定；也可分装成单次用量，2 年稳定。避免反复冻融。
用于酶切或蛋白测序分析的工作条件
缓冲液：50mM NH4HCO3 (pH 7.8) 用量：TPCK-TRYPSIN：目标蛋白＝1:20-50
反应条件：37℃，2-3 小时 活性 BAEE>10000 Unit/mg BTEE<0.1 Unit/mg
单位定义：1 BTEE 单位 = 320 ATEE 单位
【注意 】
●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。
This product is trypsin treated with TPCK, so the activity of trypsin is higher and the activity of chymotrypsin is inhibited. TPCK-trypsin can specifically cut the arginine and lysine sites in protein molecules and is one of the important tool enzymes for protein peptide analysis.
Purpose
Scientific research reagents, widely used in molecular biology, pharmacology and other scientific research, strictly prohibited for use on humans. Trypsin digests peptides using a ratio of about 1:100 to 1:20 trypsin: peptide. The typical use of this product is to remove adherent cells from culture surfaces. The concentration of trypsin required to remove cells from the cell matrix depends mainly on the cell type and culture time. Trypsin is also used for cell suspension during cell suspension culture, proteomics research, for digestion of proteins and various gel digestions. Other applications include evaluation of crystallization by membrane-based techniques, and for studies to determine whether protein folding rates and yields may be limited by the presence of kinetic traps.
| Unit Definition |
1 BTEE unit=320 ATEE units |
| Source |
Bovine pancreas |
| CAS No. |
'9002-07-7 |
| Storage conditions |
-20℃，Avoid light, moisture, sealed and dry |
| Molecular weight |
23.8 kDa |
| Solubility(25°C) |
hydrochloric acid: soluble 1 mM |
| Precautions |
Solubility is measured at room temperature, and if the temperature is too low, it may affect its solubility. |
| Others |
For your safety and health, please wear lab coats and disposable gloves when operating. | |
Recommended usage method:
1： Preparation of storage solution for cell culture related experiments: Dissolve according to the solubility in the table. If used for cell experiments, prepare the liquid and filter it with 0.22um before adding the cells.
2： Storage of liquid: It is recommended to prepare and use it immediately, as the liquid is not very stable; It can also be divided into single use quantities, stable for 2 years. Avoid repeated freezing and thawing.
Working conditions for enzyme digestion or protein sequencing analysis
Buffer solution: 50mM NH4HCO3 (pH 7.8) dosage: TPCK-TRYPSIN： Target protein=1:20-50
Reaction conditions: 37 ℃, 2-3 hours Activity BAEE>10000 Unit/mg BTEE<0.1 Unit/mg
Unit definition: 1 BTEE unit=320 ATEE units
[Attention]
Our products are non sterile packaging. If used for cell culture, please perform pre-treatment in advance to remove pyrogenic bacteria, otherwise it may cause bacterial contamination.
Our company can only provide partial information for some products, and we do not guarantee the authority of the information provided. The above data is for reference, communication, and research purposes only.
E2625545
危险品化学品经营许可证（带存储）