计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
有货
为什么选择此级别
分析标准品 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 5635 篇同行评审文献引用。
概述
EDC 是羧基和胺反应性零长度交联剂。EDC 首先与羧基发生反应，形成胺反应性 O-酰基异脲中间体，该中间体可与氨基快速反应形成酰胺键，释放异脲副产物。中间体在水性溶液中不稳定，因此两步偶联程序依赖于 N-羟基琥珀酰亚胺 (NHS) 进行稳定。如果不与胺发生反应，将导致中间体的水解、羧基的再生和 N-取代尿素的释放。副反应是形成 N-酰脲，通常仅限于位于蛋白疏水性区域的羧基。
EDC 的特点：
•反应性基团：碳二亚胺
• 反应靶标：活化羧基与氨基（伯胺）偶联
• 多种偶联策略—将 EDC 单独与靶标基团反应，或包括 NHS 或磺基-NHS，以提高反应效率或稳定活性中间体，用于后续胺类反应
• 中性键—在羧基和胺之间形成中性酰胺键
• 水溶性试剂—在水性生理缓冲液中直接加入进行反应
• 可溶性反应副产物—使用水或稀释酸洗涤即可轻松去除
• 高纯度结晶试剂—用于生成高质量、活化的衍生物
• 反应性：在 pH 值为 4.7–6.0（较优）的条件下与羧基形成活性中间体，然后中间体与伯胺发生反应
产品应用：
• 偶联肽和蛋白间的羧基与氨基基团
• 偶联半抗原与免疫原性载体蛋白（如将肽连接至 KLH）
• 将肽抗原固定在亲和纯化抗体上
• 生成 NHS 活化、胺反应性标记化合物
• 交联蛋白与羧基包被的微珠或表面
• 使用胺反应性 Sulfo-NHS 酯活化纳米微粒
• 通过 5' 磷酸基进行 DNA 标记
EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.
Characteristics of EDC:
• Reactive group:carbodiimide
• Reaction target: activates carboxyl groups to conjugate to amino groups (primary amines)
• Several conjugation strategies—react EDC alone with target groups or includecarbodiimide
• Reactivity: Forms active intermediate with carboxyl groups at pH 4.7–6.0 (optimum), then intermediate reacts with primary amines
规格
别名
阿拉丁™EDC
规格或纯度
分析标准品
英文名称
Aladdin™ EDC
储存条件
-20°C储存,干燥
运输条件
超低温运输
名称和识别符
PubChem SID
EC号
247-361-2
分子类型
小分子
INCHI
1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChi Key
FPQQSJJWHUJYPU-UHFFFAOYSA-N
Smiles
CCN=C=NCCCN(C)C.Cl
Isomeric SMILES
CCN=C=NCCCN(C)C.Cl
关联CAS
分子量
191.70
Beilstein号
5764110
Reaxy-Rn
5764110
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5764110&ln=
技术文档
高级数据
系统学分类
分类树（Taxonomy Tree）
| 界（kingdom) | 有机化合物 |
|---|---|
| 超类（Superclass) | 有机氮化合物 |
| 类（Class) | 有机氮化合物 |
| 亚类（Subclass） | 胺类 |
| 中间层级节点（Intermediate Tree Nodes） | 叔胺 |
| 直接上位类(Direct Parent) | 三烷基胺 |
| 其他上位类（Alternative Parents） | 炔丙基型1,3-偶极有机化合物 碳二亚胺 有机光子化合物 盐酸盐 碳氢化合物衍生物 |
| 分子骨架（Molecular Framework) | 脂肪族无环化合物 |
| 取代基（Substituents） | 脂肪族非环化合物 - 碳二亚胺 - 烃衍生物 - 氟氯烃 - 有机1,3-双极性化合物 - 有机氮化合物 - 丙炔型1,3-双极性有机化合物 - 三元脂肪胺 |
| 描述（Description） | 该化合物属于有机化合物中的三烷基胺类。这类化合物含有三烷基胺基团，其特征在于三个烷基基团精确地与氨基氮原子相连。 |
| 外部描述符（External Descriptors） | 暂无 |
三维结构
交互式化学结构模型
关联靶点（人）
A498
(42825 活性数据)
ACHN
(49357 活性数据)
CAKI-1
(44928 活性数据)
CCRF-CEM
(65223 活性数据)
COLO 205
(50209 活性数据)
DU-145
(51482 活性数据)
HL-60
(67320 活性数据)
HT-29
(80576 活性数据)
K562
(73714 活性数据)
KM12
(47707 活性数据)
M14
(47487 活性数据)
MCF7
(126967 活性数据)
MOLT-4
(49676 活性数据)
OVCAR-3
(48710 活性数据)
OVCAR-4
(44535 活性数据)
OVCAR-5
(45555 活性数据)
OVCAR-8
(47708 活性数据)
PC-3
(62116 活性数据)
RPMI-8226
(44974 活性数据)
RXF 393
(41971 活性数据)
SF-295
(48000 活性数据)
SK-MEL-2
(46422 活性数据)
SK-MEL-28
(48833 活性数据)
SK-MEL-5
(47095 活性数据)
SK-OV-3
(52876 活性数据)
SN12C
(47755 活性数据)
SNB-19
(46794 活性数据)
TK-10
(45540 活性数据)
U-251
(51189 活性数据)
UACC-257
(46019 活性数据)
UACC-62
(47335 活性数据)
UO-31
(46270 活性数据)
786-0
(47912 活性数据)
A549
(127892 活性数据)
NCI/ADR-RES
(33767 活性数据)
EKVX
(44102 活性数据)
NCI-H322M
(45589 活性数据)
HCT-116
(91556 活性数据)
HOP-92
(41141 活性数据)
Hs-578T
(29457 活性数据)
NCI-H460
(60772 活性数据)
SF-268
(49410 活性数据)
IGROV-1
(47897 活性数据)
LOX IMVI
(44321 活性数据)
HOP-62
(47048 活性数据)
MDA-MB-435
(38290 活性数据)
MDA-N
(28205 活性数据)
Malme-3M
(44254 活性数据)
MDA-MB-231
(73002 活性数据)
SNB-75
(44215 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
化学和物理性质
溶解性
Water
熔点
115-118°C
分子量
191.700 g/mol
XLogP3
氢键供体数Hydrogen Bond Donor Count
1
氢键受体数Hydrogen Bond Acceptor Count
3
可旋转键计数Rotatable Bond Count
5
精确质量Exact Mass
191.119 Da
单同位素质量Monoisotopic Mass
191.119 Da
拓扑极表面积Topological Polar Surface Area
28.000 Ų
重原子数Heavy Atom Count
12
形式电荷Formal Charge
0
复杂度Complexity
134.000
同位素原子数Isotope Atom Count
0
定义的原子立体中心计数Defined Atom Stereocenter Count
0
未定义的原子立体中心计数Undefined Atom Stereocenter Count
0
定义的键立体中心计数Defined Bond Stereocenter Count
0
未定义的键立体中心计数Undefined Bond Stereocenter Count
0
所有立体化学键的总数The total count of all stereochemical bonds
0
共价键合单元计数Covalently-Bonded Unit Count
2
安全和危险性（GHS）
信号词
危险
危险声明
H302: 吞食有害
H311: 皮肤接触有毒
H315: 引起皮肤刺激
H317: 可能引起皮肤过敏反应
H319: 引起严重眼睛刺激
H373: 通过长时间或反复暴露对器官造成损害
H410: 对水生生物有剧毒并具有长期持续影响
WGK Germany
3
RTECS
FF2200000
个人防护装备
dust mask type N95 (US),Eyeshields,Gloves
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 D2613150 |
分析证书 | A638729 |
| D2613152 |
分析证书 | A638729 |
| D2613153 |
分析证书 | A638729 |
| D2613154 |
分析证书 | A638729 |
| D2613171 |
分析证书 | A638729 |
| H2420498 |
分析证书 | A638729 |
| H2420556 |
分析证书 | A638729 |
| I2511038 |
分析证书 | A638729 |
| J2422272 |
分析证书 | A638729 |
| J2429232 |
分析证书 | A638729 |
| K2407241 |
分析证书 | A638729 |
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此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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